Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS19610 and BPHYT_RS19615 are separated by 173 nucleotides BPHYT_RS19615 and BPHYT_RS19620 are separated by 196 nucleotides BPHYT_RS19620 and BPHYT_RS19625 are separated by 1 nucleotides BPHYT_RS19625 and BPHYT_RS19630 overlap by 23 nucleotides
BPHYT_RS19610: BPHYT_RS19610 - general secretion pathway protein GspC, at 4,414,676 to 4,415,089
_RS19610
BPHYT_RS19615: BPHYT_RS19615 - general secretion pathway protein G, at 4,415,263 to 4,415,727
_RS19615
BPHYT_RS19620: BPHYT_RS19620 - general secretion pathway protein GspH, at 4,415,924 to 4,416,415
_RS19620
BPHYT_RS19625: BPHYT_RS19625 - general secretion pathway protein I, at 4,416,417 to 4,416,833
_RS19625
BPHYT_RS19630: BPHYT_RS19630 - general secretion pathway protein J, at 4,416,811 to 4,417,497
_RS19630
Position (kb) 4415
4416
4417 Strain fitness (log2 ratio) -2
-1
0
1 at 4414.931 kb on + strand, within BPHYT_RS19610 at 4415.022 kb on + strand, within BPHYT_RS19610 at 4415.102 kb on - strand at 4415.102 kb on - strand at 4415.108 kb on + strand at 4415.169 kb on - strand at 4415.262 kb on + strand at 4415.262 kb on + strand at 4415.262 kb on + strand at 4415.263 kb on - strand at 4415.833 kb on + strand at 4415.833 kb on + strand at 4415.834 kb on - strand at 4416.209 kb on - strand, within BPHYT_RS19620 at 4416.309 kb on + strand, within BPHYT_RS19620 at 4416.310 kb on - strand, within BPHYT_RS19620 at 4416.310 kb on - strand, within BPHYT_RS19620 at 4416.611 kb on + strand, within BPHYT_RS19625 at 4416.611 kb on + strand, within BPHYT_RS19625 at 4416.612 kb on - strand, within BPHYT_RS19625 at 4417.156 kb on + strand, within BPHYT_RS19630 at 4417.290 kb on + strand, within BPHYT_RS19630 at 4417.291 kb on - strand, within BPHYT_RS19630 at 4417.306 kb on + strand, within BPHYT_RS19630 at 4417.306 kb on + strand, within BPHYT_RS19630 at 4417.375 kb on - strand, within BPHYT_RS19630
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 4,414,931 + BPHYT_RS19610 0.62 -1.1 4,415,022 + BPHYT_RS19610 0.84 -1.3 4,415,102 - +0.0 4,415,102 - -0.3 4,415,108 + -0.6 4,415,169 - -1.6 4,415,262 + +0.3 4,415,262 + -0.6 4,415,262 + -0.2 4,415,263 - +0.5 4,415,833 + +0.3 4,415,833 + -1.1 4,415,834 - -1.2 4,416,209 - BPHYT_RS19620 0.58 +0.9 4,416,309 + BPHYT_RS19620 0.78 -1.4 4,416,310 - BPHYT_RS19620 0.78 -2.6 4,416,310 - BPHYT_RS19620 0.78 -1.4 4,416,611 + BPHYT_RS19625 0.47 -0.3 4,416,611 + BPHYT_RS19625 0.47 -1.0 4,416,612 - BPHYT_RS19625 0.47 +0.3 4,417,156 + BPHYT_RS19630 0.50 +0.3 4,417,290 + BPHYT_RS19630 0.70 +0.2 4,417,291 - BPHYT_RS19630 0.70 -0.2 4,417,306 + BPHYT_RS19630 0.72 -0.2 4,417,306 + BPHYT_RS19630 0.72 -1.2 4,417,375 - BPHYT_RS19630 0.82 -1.2
Or see this region's nucleotide sequence