Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS16675 and BPHYT_RS16680 are separated by 184 nucleotides BPHYT_RS16680 and BPHYT_RS16685 are separated by 119 nucleotides BPHYT_RS16685 and BPHYT_RS16690 overlap by 4 nucleotides
BPHYT_RS16675: BPHYT_RS16675 - dihydroorotase, at 3,775,534 to 3,776,595
_RS16675
BPHYT_RS16680: BPHYT_RS16680 - glutamine amidotransferase, at 3,776,780 to 3,777,697
_RS16680
BPHYT_RS16685: BPHYT_RS16685 - arginine ABC transporter ATP-binding protein, at 3,777,817 to 3,778,542
_RS16685
BPHYT_RS16690: BPHYT_RS16690 - glutamate/aspartate transporter permease GltK, at 3,778,539 to 3,779,216
_RS16690
Position (kb)
3776
3777
3778 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3775.920 kb on - strand, within BPHYT_RS16675 at 3776.027 kb on + strand, within BPHYT_RS16675 at 3776.184 kb on + strand, within BPHYT_RS16675 at 3776.633 kb on + strand at 3776.633 kb on + strand at 3776.633 kb on + strand at 3776.633 kb on + strand at 3776.634 kb on - strand at 3776.661 kb on + strand at 3776.706 kb on - strand at 3776.706 kb on - strand at 3776.706 kb on - strand at 3776.706 kb on - strand at 3776.706 kb on - strand at 3777.102 kb on - strand, within BPHYT_RS16680 at 3777.335 kb on + strand, within BPHYT_RS16680 at 3777.335 kb on + strand, within BPHYT_RS16680 at 3777.336 kb on - strand, within BPHYT_RS16680 at 3777.336 kb on - strand, within BPHYT_RS16680 at 3777.336 kb on - strand, within BPHYT_RS16680 at 3777.338 kb on + strand, within BPHYT_RS16680 at 3777.338 kb on + strand, within BPHYT_RS16680 at 3777.338 kb on + strand, within BPHYT_RS16680 at 3777.338 kb on + strand, within BPHYT_RS16680 at 3777.339 kb on - strand, within BPHYT_RS16680 at 3777.410 kb on + strand, within BPHYT_RS16680 at 3777.410 kb on + strand, within BPHYT_RS16680 at 3777.410 kb on + strand, within BPHYT_RS16680 at 3777.411 kb on - strand, within BPHYT_RS16680 at 3777.809 kb on + strand at 3778.603 kb on + strand at 3778.624 kb on - strand, within BPHYT_RS16690 at 3778.624 kb on - strand, within BPHYT_RS16690 at 3778.624 kb on - strand, within BPHYT_RS16690 at 3778.624 kb on - strand, within BPHYT_RS16690 at 3778.658 kb on + strand, within BPHYT_RS16690
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 3,775,920 - BPHYT_RS16675 0.36 +0.5 3,776,027 + BPHYT_RS16675 0.46 +2.4 3,776,184 + BPHYT_RS16675 0.61 +1.4 3,776,633 + -0.2 3,776,633 + +3.9 3,776,633 + +1.4 3,776,633 + -0.5 3,776,634 - +0.5 3,776,661 + -0.2 3,776,706 - +1.2 3,776,706 - -1.3 3,776,706 - -2.2 3,776,706 - +0.2 3,776,706 - +0.9 3,777,102 - BPHYT_RS16680 0.35 -0.3 3,777,335 + BPHYT_RS16680 0.60 -0.2 3,777,335 + BPHYT_RS16680 0.60 -0.8 3,777,336 - BPHYT_RS16680 0.61 -0.9 3,777,336 - BPHYT_RS16680 0.61 -1.4 3,777,336 - BPHYT_RS16680 0.61 -0.1 3,777,338 + BPHYT_RS16680 0.61 -0.3 3,777,338 + BPHYT_RS16680 0.61 -0.5 3,777,338 + BPHYT_RS16680 0.61 -0.7 3,777,338 + BPHYT_RS16680 0.61 +2.4 3,777,339 - BPHYT_RS16680 0.61 -0.8 3,777,410 + BPHYT_RS16680 0.69 +1.9 3,777,410 + BPHYT_RS16680 0.69 -0.8 3,777,410 + BPHYT_RS16680 0.69 -0.8 3,777,411 - BPHYT_RS16680 0.69 -0.1 3,777,809 + -0.1 3,778,603 + -1.9 3,778,624 - BPHYT_RS16690 0.13 +0.4 3,778,624 - BPHYT_RS16690 0.13 -1.1 3,778,624 - BPHYT_RS16690 0.13 +0.3 3,778,624 - BPHYT_RS16690 0.13 -1.1 3,778,658 + BPHYT_RS16690 0.18 -0.8
Or see this region's nucleotide sequence