Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14990 and BPHYT_RS14995 are separated by 14 nucleotides BPHYT_RS14995 and BPHYT_RS15000 are separated by 8 nucleotides BPHYT_RS15000 and BPHYT_RS15005 are separated by 204 nucleotides
BPHYT_RS14990: BPHYT_RS14990 - formate dehydrogenase subunit beta, at 3,406,554 to 3,408,116
_RS14990
BPHYT_RS14995: BPHYT_RS14995 - formate dehydrogenase subunit alpha, at 3,408,131 to 3,411,079
_RS14995
BPHYT_RS15000: BPHYT_RS15000 - formate dehydrogenase subunit delta, at 3,411,088 to 3,411,339
_RS15000
BPHYT_RS15005: BPHYT_RS15005 - FAD-binding monooxygenase, at 3,411,544 to 3,413,268
_RS15005
Position (kb)
3408
3409
3410
3411
3412 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 3407.148 kb on + strand, within BPHYT_RS14990 at 3407.148 kb on + strand, within BPHYT_RS14990 at 3407.148 kb on + strand, within BPHYT_RS14990 at 3407.148 kb on + strand, within BPHYT_RS14990 at 3407.149 kb on - strand, within BPHYT_RS14990 at 3407.149 kb on - strand, within BPHYT_RS14990 at 3407.208 kb on - strand, within BPHYT_RS14990 at 3407.208 kb on - strand, within BPHYT_RS14990 at 3407.211 kb on + strand, within BPHYT_RS14990 at 3407.211 kb on + strand, within BPHYT_RS14990 at 3407.211 kb on + strand, within BPHYT_RS14990 at 3407.212 kb on - strand, within BPHYT_RS14990 at 3407.212 kb on - strand, within BPHYT_RS14990 at 3407.352 kb on + strand, within BPHYT_RS14990 at 3407.352 kb on + strand, within BPHYT_RS14990 at 3407.353 kb on - strand, within BPHYT_RS14990 at 3407.357 kb on - strand, within BPHYT_RS14990 at 3407.397 kb on - strand, within BPHYT_RS14990 at 3407.526 kb on + strand, within BPHYT_RS14990 at 3407.715 kb on + strand, within BPHYT_RS14990 at 3407.715 kb on + strand, within BPHYT_RS14990 at 3407.715 kb on + strand, within BPHYT_RS14990 at 3408.088 kb on + strand at 3408.088 kb on + strand at 3408.089 kb on - strand at 3408.089 kb on - strand at 3408.089 kb on - strand at 3408.089 kb on - strand at 3408.187 kb on + strand at 3408.360 kb on + strand at 3408.361 kb on - strand at 3408.361 kb on - strand at 3408.539 kb on + strand, within BPHYT_RS14995 at 3408.539 kb on + strand, within BPHYT_RS14995 at 3408.539 kb on + strand, within BPHYT_RS14995 at 3408.539 kb on + strand at 3408.539 kb on + strand, within BPHYT_RS14995 at 3408.539 kb on + strand, within BPHYT_RS14995 at 3408.539 kb on + strand, within BPHYT_RS14995 at 3408.539 kb on + strand, within BPHYT_RS14995 at 3408.539 kb on + strand, within BPHYT_RS14995 at 3408.540 kb on - strand, within BPHYT_RS14995 at 3408.540 kb on - strand, within BPHYT_RS14995 at 3408.540 kb on - strand, within BPHYT_RS14995 at 3408.540 kb on - strand, within BPHYT_RS14995 at 3408.541 kb on + strand, within BPHYT_RS14995 at 3408.541 kb on + strand, within BPHYT_RS14995 at 3408.541 kb on + strand at 3409.173 kb on - strand, within BPHYT_RS14995 at 3409.173 kb on - strand, within BPHYT_RS14995 at 3409.234 kb on + strand, within BPHYT_RS14995 at 3409.234 kb on + strand, within BPHYT_RS14995 at 3409.234 kb on + strand, within BPHYT_RS14995 at 3409.265 kb on + strand, within BPHYT_RS14995 at 3409.265 kb on + strand, within BPHYT_RS14995 at 3409.266 kb on - strand, within BPHYT_RS14995 at 3409.266 kb on - strand at 3409.266 kb on - strand, within BPHYT_RS14995 at 3409.266 kb on - strand, within BPHYT_RS14995 at 3409.423 kb on + strand, within BPHYT_RS14995 at 3409.790 kb on + strand, within BPHYT_RS14995 at 3409.791 kb on - strand, within BPHYT_RS14995 at 3409.791 kb on - strand, within BPHYT_RS14995 at 3409.820 kb on + strand, within BPHYT_RS14995 at 3410.129 kb on + strand, within BPHYT_RS14995 at 3410.130 kb on - strand, within BPHYT_RS14995 at 3410.130 kb on - strand, within BPHYT_RS14995 at 3410.246 kb on + strand, within BPHYT_RS14995 at 3410.247 kb on - strand, within BPHYT_RS14995 at 3410.247 kb on - strand, within BPHYT_RS14995 at 3410.247 kb on - strand, within BPHYT_RS14995 at 3410.247 kb on - strand, within BPHYT_RS14995 at 3410.293 kb on + strand, within BPHYT_RS14995 at 3410.294 kb on - strand, within BPHYT_RS14995 at 3410.414 kb on + strand, within BPHYT_RS14995 at 3410.675 kb on + strand, within BPHYT_RS14995 at 3410.914 kb on + strand at 3410.967 kb on + strand at 3410.968 kb on - strand at 3410.968 kb on - strand at 3411.338 kb on - strand at 3411.650 kb on + strand at 3411.651 kb on - strand at 3411.651 kb on - strand at 3411.671 kb on + strand at 3411.671 kb on + strand at 3411.672 kb on - strand at 3411.672 kb on - strand at 3411.672 kb on - strand at 3411.696 kb on - strand at 3411.696 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 3,407,148 + BPHYT_RS14990 0.38 +0.1 3,407,148 + BPHYT_RS14990 0.38 -0.4 3,407,148 + BPHYT_RS14990 0.38 +1.7 3,407,148 + BPHYT_RS14990 0.38 -0.6 3,407,149 - BPHYT_RS14990 0.38 -0.6 3,407,149 - BPHYT_RS14990 0.38 -0.8 3,407,208 - BPHYT_RS14990 0.42 -0.2 3,407,208 - BPHYT_RS14990 0.42 +0.5 3,407,211 + BPHYT_RS14990 0.42 +0.0 3,407,211 + BPHYT_RS14990 0.42 +2.0 3,407,211 + BPHYT_RS14990 0.42 -0.3 3,407,212 - BPHYT_RS14990 0.42 +1.0 3,407,212 - BPHYT_RS14990 0.42 +5.0 3,407,352 + BPHYT_RS14990 0.51 -0.4 3,407,352 + BPHYT_RS14990 0.51 +3.1 3,407,353 - BPHYT_RS14990 0.51 +0.3 3,407,357 - BPHYT_RS14990 0.51 -1.5 3,407,397 - BPHYT_RS14990 0.54 -0.1 3,407,526 + BPHYT_RS14990 0.62 -0.6 3,407,715 + BPHYT_RS14990 0.74 +1.0 3,407,715 + BPHYT_RS14990 0.74 +0.8 3,407,715 + BPHYT_RS14990 0.74 +0.8 3,408,088 + -1.4 3,408,088 + +1.0 3,408,089 - +1.6 3,408,089 - +0.1 3,408,089 - -0.4 3,408,089 - +0.0 3,408,187 + -0.0 3,408,360 + +0.7 3,408,361 - +0.6 3,408,361 - +0.3 3,408,539 + BPHYT_RS14995 0.14 -1.4 3,408,539 + BPHYT_RS14995 0.14 -1.3 3,408,539 + BPHYT_RS14995 0.14 +1.4 3,408,539 + +1.4 3,408,539 + BPHYT_RS14995 0.14 -0.0 3,408,539 + BPHYT_RS14995 0.14 -1.4 3,408,539 + BPHYT_RS14995 0.14 +0.4 3,408,539 + BPHYT_RS14995 0.14 -0.5 3,408,539 + BPHYT_RS14995 0.14 -0.2 3,408,540 - BPHYT_RS14995 0.14 -0.6 3,408,540 - BPHYT_RS14995 0.14 +0.4 3,408,540 - BPHYT_RS14995 0.14 -1.4 3,408,540 - BPHYT_RS14995 0.14 -0.3 3,408,541 + BPHYT_RS14995 0.14 -1.2 3,408,541 + BPHYT_RS14995 0.14 -0.5 3,408,541 + +0.8 3,409,173 - BPHYT_RS14995 0.35 -2.4 3,409,173 - BPHYT_RS14995 0.35 +0.1 3,409,234 + BPHYT_RS14995 0.37 -0.7 3,409,234 + BPHYT_RS14995 0.37 -1.4 3,409,234 + BPHYT_RS14995 0.37 -0.7 3,409,265 + BPHYT_RS14995 0.38 -0.1 3,409,265 + BPHYT_RS14995 0.38 -0.0 3,409,266 - BPHYT_RS14995 0.38 +0.7 3,409,266 - +2.4 3,409,266 - BPHYT_RS14995 0.38 -0.9 3,409,266 - BPHYT_RS14995 0.38 -0.5 3,409,423 + BPHYT_RS14995 0.44 -1.5 3,409,790 + BPHYT_RS14995 0.56 -1.1 3,409,791 - BPHYT_RS14995 0.56 -1.1 3,409,791 - BPHYT_RS14995 0.56 -0.9 3,409,820 + BPHYT_RS14995 0.57 -0.2 3,410,129 + BPHYT_RS14995 0.68 -0.8 3,410,130 - BPHYT_RS14995 0.68 +0.5 3,410,130 - BPHYT_RS14995 0.68 +2.6 3,410,246 + BPHYT_RS14995 0.72 +0.4 3,410,247 - BPHYT_RS14995 0.72 +0.3 3,410,247 - BPHYT_RS14995 0.72 -0.6 3,410,247 - BPHYT_RS14995 0.72 -0.2 3,410,247 - BPHYT_RS14995 0.72 +0.1 3,410,293 + BPHYT_RS14995 0.73 -2.3 3,410,294 - BPHYT_RS14995 0.73 -0.2 3,410,414 + BPHYT_RS14995 0.77 +3.4 3,410,675 + BPHYT_RS14995 0.86 -0.6 3,410,914 + +0.2 3,410,967 + +0.1 3,410,968 - +0.4 3,410,968 - -1.6 3,411,338 - -0.1 3,411,650 + -0.4 3,411,651 - +0.2 3,411,651 - +0.2 3,411,671 + -0.9 3,411,671 + +0.4 3,411,672 - -0.4 3,411,672 - -0.4 3,411,672 - +0.2 3,411,696 - -0.4 3,411,696 - +0.3
Or see this region's nucleotide sequence