Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14520 and BPHYT_RS14525 are separated by 41 nucleotides BPHYT_RS14525 and BPHYT_RS14530 are separated by 74 nucleotides BPHYT_RS14530 and BPHYT_RS14535 are separated by 141 nucleotides
BPHYT_RS14520: BPHYT_RS14520 - 2-hydroxyacid dehydrogenase, at 3,310,268 to 3,311,257
_RS14520
BPHYT_RS14525: BPHYT_RS14525 - sodium:proton antiporter, at 3,311,299 to 3,312,729
_RS14525
BPHYT_RS14530: BPHYT_RS14530 - membrane protein, at 3,312,804 to 3,313,466
_RS14530
BPHYT_RS14535: BPHYT_RS14535 - nicotinate phosphoribosyltransferase, at 3,313,608 to 3,314,804
_RS14535
Position (kb)
3311
3312
3313 Strain fitness (log2 ratio)
-2
-1
0
1 at 3310.515 kb on - strand, within BPHYT_RS14520 at 3310.635 kb on + strand, within BPHYT_RS14520 at 3310.636 kb on - strand, within BPHYT_RS14520 at 3310.636 kb on - strand, within BPHYT_RS14520 at 3310.643 kb on + strand, within BPHYT_RS14520 at 3310.644 kb on - strand, within BPHYT_RS14520 at 3310.688 kb on + strand, within BPHYT_RS14520 at 3310.689 kb on - strand, within BPHYT_RS14520 at 3310.689 kb on - strand, within BPHYT_RS14520 at 3310.692 kb on + strand, within BPHYT_RS14520 at 3310.692 kb on + strand, within BPHYT_RS14520 at 3310.693 kb on - strand, within BPHYT_RS14520 at 3310.693 kb on - strand, within BPHYT_RS14520 at 3310.747 kb on - strand, within BPHYT_RS14520 at 3310.747 kb on - strand, within BPHYT_RS14520 at 3310.747 kb on - strand, within BPHYT_RS14520 at 3310.860 kb on + strand, within BPHYT_RS14520 at 3310.860 kb on + strand, within BPHYT_RS14520 at 3310.860 kb on + strand, within BPHYT_RS14520 at 3310.861 kb on - strand, within BPHYT_RS14520 at 3311.034 kb on + strand, within BPHYT_RS14520 at 3311.035 kb on - strand, within BPHYT_RS14520 at 3311.048 kb on - strand, within BPHYT_RS14520 at 3311.197 kb on - strand at 3311.197 kb on - strand at 3311.271 kb on - strand at 3311.364 kb on - strand at 3311.442 kb on - strand, within BPHYT_RS14525 at 3311.442 kb on - strand, within BPHYT_RS14525 at 3311.442 kb on - strand, within BPHYT_RS14525 at 3311.442 kb on - strand, within BPHYT_RS14525 at 3311.442 kb on - strand, within BPHYT_RS14525 at 3311.442 kb on - strand, within BPHYT_RS14525 at 3311.552 kb on + strand, within BPHYT_RS14525 at 3311.552 kb on + strand, within BPHYT_RS14525 at 3311.552 kb on + strand, within BPHYT_RS14525 at 3311.553 kb on - strand, within BPHYT_RS14525 at 3311.603 kb on + strand, within BPHYT_RS14525 at 3311.793 kb on + strand, within BPHYT_RS14525 at 3311.794 kb on - strand, within BPHYT_RS14525 at 3312.050 kb on + strand, within BPHYT_RS14525 at 3312.051 kb on - strand, within BPHYT_RS14525 at 3312.051 kb on - strand, within BPHYT_RS14525 at 3312.051 kb on - strand, within BPHYT_RS14525 at 3312.204 kb on + strand, within BPHYT_RS14525 at 3312.217 kb on + strand, within BPHYT_RS14525 at 3312.218 kb on - strand, within BPHYT_RS14525 at 3312.218 kb on - strand, within BPHYT_RS14525 at 3312.425 kb on - strand, within BPHYT_RS14525 at 3312.428 kb on + strand, within BPHYT_RS14525 at 3312.428 kb on + strand, within BPHYT_RS14525 at 3312.429 kb on - strand, within BPHYT_RS14525 at 3312.479 kb on + strand, within BPHYT_RS14525 at 3312.480 kb on - strand, within BPHYT_RS14525 at 3312.574 kb on + strand, within BPHYT_RS14525 at 3312.574 kb on + strand, within BPHYT_RS14525 at 3312.730 kb on - strand at 3312.730 kb on - strand at 3312.748 kb on - strand at 3313.033 kb on + strand, within BPHYT_RS14530 at 3313.034 kb on - strand, within BPHYT_RS14530 at 3313.034 kb on - strand, within BPHYT_RS14530 at 3313.060 kb on - strand, within BPHYT_RS14530 at 3313.107 kb on + strand, within BPHYT_RS14530 at 3313.222 kb on + strand, within BPHYT_RS14530 at 3313.363 kb on + strand, within BPHYT_RS14530 at 3313.493 kb on + strand at 3313.494 kb on - strand at 3313.494 kb on - strand at 3313.494 kb on - strand at 3313.498 kb on + strand at 3313.510 kb on + strand at 3313.510 kb on + strand at 3313.511 kb on - strand at 3313.511 kb on - strand at 3313.512 kb on + strand at 3313.512 kb on + strand at 3313.553 kb on - strand at 3313.578 kb on + strand at 3313.578 kb on + strand at 3313.579 kb on - strand at 3313.584 kb on - strand at 3313.661 kb on - strand at 3313.669 kb on + strand at 3313.670 kb on - strand at 3313.670 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 3,310,515 - BPHYT_RS14520 0.25 -1.5 3,310,635 + BPHYT_RS14520 0.37 -0.4 3,310,636 - BPHYT_RS14520 0.37 -1.0 3,310,636 - BPHYT_RS14520 0.37 +0.5 3,310,643 + BPHYT_RS14520 0.38 -1.8 3,310,644 - BPHYT_RS14520 0.38 +0.4 3,310,688 + BPHYT_RS14520 0.42 -0.4 3,310,689 - BPHYT_RS14520 0.43 +0.7 3,310,689 - BPHYT_RS14520 0.43 -1.3 3,310,692 + BPHYT_RS14520 0.43 +0.5 3,310,692 + BPHYT_RS14520 0.43 -0.7 3,310,693 - BPHYT_RS14520 0.43 +1.2 3,310,693 - BPHYT_RS14520 0.43 -1.4 3,310,747 - BPHYT_RS14520 0.48 -0.9 3,310,747 - BPHYT_RS14520 0.48 -1.0 3,310,747 - BPHYT_RS14520 0.48 -0.3 3,310,860 + BPHYT_RS14520 0.60 -0.0 3,310,860 + BPHYT_RS14520 0.60 -1.4 3,310,860 + BPHYT_RS14520 0.60 +0.9 3,310,861 - BPHYT_RS14520 0.60 -0.2 3,311,034 + BPHYT_RS14520 0.77 -0.4 3,311,035 - BPHYT_RS14520 0.77 -0.4 3,311,048 - BPHYT_RS14520 0.79 +0.1 3,311,197 - +1.1 3,311,197 - -0.0 3,311,271 - +0.3 3,311,364 - +0.6 3,311,442 - BPHYT_RS14525 0.10 -0.8 3,311,442 - BPHYT_RS14525 0.10 +0.7 3,311,442 - BPHYT_RS14525 0.10 -0.0 3,311,442 - BPHYT_RS14525 0.10 +0.3 3,311,442 - BPHYT_RS14525 0.10 +0.8 3,311,442 - BPHYT_RS14525 0.10 +1.7 3,311,552 + BPHYT_RS14525 0.18 -0.8 3,311,552 + BPHYT_RS14525 0.18 -0.4 3,311,552 + BPHYT_RS14525 0.18 -2.1 3,311,553 - BPHYT_RS14525 0.18 -1.6 3,311,603 + BPHYT_RS14525 0.21 -1.6 3,311,793 + BPHYT_RS14525 0.35 -0.8 3,311,794 - BPHYT_RS14525 0.35 -0.5 3,312,050 + BPHYT_RS14525 0.52 -0.8 3,312,051 - BPHYT_RS14525 0.53 -1.3 3,312,051 - BPHYT_RS14525 0.53 +0.9 3,312,051 - BPHYT_RS14525 0.53 -0.9 3,312,204 + BPHYT_RS14525 0.63 +0.4 3,312,217 + BPHYT_RS14525 0.64 -0.4 3,312,218 - BPHYT_RS14525 0.64 +0.6 3,312,218 - BPHYT_RS14525 0.64 -0.7 3,312,425 - BPHYT_RS14525 0.79 +1.4 3,312,428 + BPHYT_RS14525 0.79 -0.1 3,312,428 + BPHYT_RS14525 0.79 -0.7 3,312,429 - BPHYT_RS14525 0.79 -1.5 3,312,479 + BPHYT_RS14525 0.82 -0.5 3,312,480 - BPHYT_RS14525 0.83 -0.6 3,312,574 + BPHYT_RS14525 0.89 -1.8 3,312,574 + BPHYT_RS14525 0.89 -0.7 3,312,730 - -0.2 3,312,730 - -0.6 3,312,748 - +0.1 3,313,033 + BPHYT_RS14530 0.35 -0.8 3,313,034 - BPHYT_RS14530 0.35 +1.3 3,313,034 - BPHYT_RS14530 0.35 +0.4 3,313,060 - BPHYT_RS14530 0.39 +1.6 3,313,107 + BPHYT_RS14530 0.46 -0.1 3,313,222 + BPHYT_RS14530 0.63 -0.4 3,313,363 + BPHYT_RS14530 0.84 -0.6 3,313,493 + +0.2 3,313,494 - -1.5 3,313,494 - +0.6 3,313,494 - -0.7 3,313,498 + -1.4 3,313,510 + -1.8 3,313,510 + +0.6 3,313,511 - +0.4 3,313,511 - +0.0 3,313,512 + -1.6 3,313,512 + +0.2 3,313,553 - -0.3 3,313,578 + -0.0 3,313,578 + +0.3 3,313,579 - +1.0 3,313,584 - -0.1 3,313,661 - +0.4 3,313,669 + +0.2 3,313,670 - -1.1 3,313,670 - -1.3
Or see this region's nucleotide sequence