Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13600 and BPHYT_RS13605 are separated by 741 nucleotides BPHYT_RS13605 and BPHYT_RS13610 are separated by 63 nucleotides BPHYT_RS13610 and BPHYT_RS13615 are separated by 46 nucleotides
BPHYT_RS13600: BPHYT_RS13600 - thioesterase, at 3,101,849 to 3,102,421
_RS13600
BPHYT_RS13605: BPHYT_RS13605 - tRNA-Pro, at 3,103,163 to 3,103,239
_RS13605
BPHYT_RS13610: BPHYT_RS13610 - MerR family transcriptional regulator, at 3,103,303 to 3,103,716
_RS13610
BPHYT_RS13615: BPHYT_RS13615 - integration host factor subunit alpha, at 3,103,763 to 3,104,173
_RS13615
Position (kb)
3103
3104 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3102.306 kb on - strand, within BPHYT_RS13600 at 3102.350 kb on - strand, within BPHYT_RS13600 at 3102.390 kb on - strand at 3102.390 kb on - strand at 3102.474 kb on + strand at 3102.517 kb on + strand at 3102.517 kb on + strand at 3102.519 kb on + strand at 3102.529 kb on - strand at 3102.643 kb on + strand at 3102.761 kb on + strand at 3102.780 kb on + strand at 3102.780 kb on + strand at 3102.781 kb on - strand at 3102.837 kb on - strand at 3102.905 kb on + strand at 3102.921 kb on - strand at 3103.155 kb on - strand at 3103.214 kb on - strand, within BPHYT_RS13605 at 3103.256 kb on - strand at 3103.256 kb on - strand at 3103.325 kb on - strand at 3103.500 kb on - strand, within BPHYT_RS13610 at 3103.548 kb on - strand, within BPHYT_RS13610 at 3103.551 kb on - strand, within BPHYT_RS13610 at 3103.551 kb on - strand, within BPHYT_RS13610 at 3103.665 kb on - strand, within BPHYT_RS13610 at 3104.177 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 3,102,306 - BPHYT_RS13600 0.80 +0.5 3,102,350 - BPHYT_RS13600 0.87 -1.5 3,102,390 - -0.3 3,102,390 - +1.6 3,102,474 + -0.1 3,102,517 + -0.9 3,102,517 + +0.3 3,102,519 + -2.5 3,102,529 - +0.1 3,102,643 + +0.5 3,102,761 + -0.6 3,102,780 + -1.5 3,102,780 + +0.6 3,102,781 - -0.7 3,102,837 - +0.9 3,102,905 + -0.1 3,102,921 - +1.3 3,103,155 - -1.8 3,103,214 - BPHYT_RS13605 0.66 -2.4 3,103,256 - +0.3 3,103,256 - +0.5 3,103,325 - +2.4 3,103,500 - BPHYT_RS13610 0.48 -1.6 3,103,548 - BPHYT_RS13610 0.59 -0.1 3,103,551 - BPHYT_RS13610 0.60 +2.4 3,103,551 - BPHYT_RS13610 0.60 +1.4 3,103,665 - BPHYT_RS13610 0.87 -0.9 3,104,177 + +1.8
Or see this region's nucleotide sequence