Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS11110 and BPHYT_RS11115 are separated by 191 nucleotides BPHYT_RS11115 and BPHYT_RS11120 are separated by 82 nucleotides BPHYT_RS11120 and BPHYT_RS11125 are separated by 239 nucleotides BPHYT_RS11125 and BPHYT_RS11130 are separated by 36 nucleotides
BPHYT_RS11110: BPHYT_RS11110 - chemotaxis protein CheY, at 2,524,442 to 2,526,415
_RS11110
BPHYT_RS11115: BPHYT_RS11115 - hypothetical protein, at 2,526,607 to 2,526,849
_RS11115
BPHYT_RS11120: BPHYT_RS11120 - ferredoxin--NADP reductase, at 2,526,932 to 2,527,981
_RS11120
BPHYT_RS11125: BPHYT_RS11125 - alkylhydroperoxidase, at 2,528,221 to 2,528,658
_RS11125
BPHYT_RS11130: BPHYT_RS11130 - chemotaxis protein, at 2,528,695 to 2,530,269
_RS11130
Position (kb)
2526
2527
2528 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2526.177 kb on + strand, within BPHYT_RS11110 at 2526.321 kb on + strand at 2526.429 kb on - strand at 2527.015 kb on - strand at 2527.020 kb on + strand at 2528.002 kb on + strand at 2528.003 kb on - strand at 2528.003 kb on - strand at 2528.243 kb on - strand at 2528.369 kb on - strand, within BPHYT_RS11125 at 2528.823 kb on + strand at 2528.920 kb on - strand, within BPHYT_RS11130
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 2,526,177 + BPHYT_RS11110 0.88 +0.2 2,526,321 + -0.1 2,526,429 - +1.0 2,527,015 - -0.4 2,527,020 + +1.8 2,528,002 + +1.4 2,528,003 - -1.5 2,528,003 - +0.8 2,528,243 - -1.7 2,528,369 - BPHYT_RS11125 0.34 -1.8 2,528,823 + +0.5 2,528,920 - BPHYT_RS11130 0.14 -0.8
Or see this region's nucleotide sequence