Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS08590 and BPHYT_RS08595 are separated by 4 nucleotides BPHYT_RS08595 and BPHYT_RS08600 are separated by 31 nucleotides BPHYT_RS08600 and BPHYT_RS08605 are separated by 72 nucleotides BPHYT_RS08605 and BPHYT_RS08610 are separated by 287 nucleotides BPHYT_RS08610 and BPHYT_RS08615 are separated by 176 nucleotides
BPHYT_RS08590: BPHYT_RS08590 - hypothetical protein, at 1,962,030 to 1,962,527
_RS08590
BPHYT_RS08595: BPHYT_RS08595 - multidrug transporter, at 1,962,532 to 1,962,870
_RS08595
BPHYT_RS08600: BPHYT_RS08600 - hypothetical protein, at 1,962,902 to 1,963,297
_RS08600
BPHYT_RS08605: BPHYT_RS08605 - hypothetical protein, at 1,963,370 to 1,963,561
_RS08605
BPHYT_RS08610: BPHYT_RS08610 - hypothetical protein, at 1,963,849 to 1,964,127
_RS08610
BPHYT_RS08615: BPHYT_RS08615 - hypothetical protein, at 1,964,304 to 1,964,588
_RS08615
Position (kb) 1963
1964 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1962.498 kb on + strand at 1962.499 kb on - strand at 1962.670 kb on + strand, within BPHYT_RS08595 at 1962.671 kb on - strand, within BPHYT_RS08595 at 1962.803 kb on + strand, within BPHYT_RS08595 at 1962.880 kb on + strand at 1962.903 kb on + strand at 1962.903 kb on + strand at 1962.904 kb on - strand at 1962.904 kb on - strand at 1962.904 kb on - strand at 1963.012 kb on - strand, within BPHYT_RS08600 at 1963.561 kb on + strand at 1963.562 kb on - strand at 1963.684 kb on + strand at 1963.685 kb on - strand at 1963.705 kb on + strand at 1963.705 kb on + strand at 1963.709 kb on + strand at 1963.710 kb on - strand at 1963.830 kb on + strand at 1963.885 kb on + strand, within BPHYT_RS08610 at 1963.886 kb on - strand, within BPHYT_RS08610 at 1963.886 kb on - strand, within BPHYT_RS08610 at 1963.960 kb on + strand, within BPHYT_RS08610 at 1963.960 kb on + strand, within BPHYT_RS08610 at 1963.961 kb on - strand, within BPHYT_RS08610 at 1964.010 kb on + strand, within BPHYT_RS08610 at 1964.011 kb on - strand, within BPHYT_RS08610 at 1964.099 kb on - strand, within BPHYT_RS08610 at 1964.232 kb on - strand at 1964.439 kb on + strand, within BPHYT_RS08615
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 1,962,498 + -3.2 1,962,499 - -0.4 1,962,670 + BPHYT_RS08595 0.41 +1.4 1,962,671 - BPHYT_RS08595 0.41 -0.2 1,962,803 + BPHYT_RS08595 0.80 -0.1 1,962,880 + +0.8 1,962,903 + -0.1 1,962,903 + +0.3 1,962,904 - +1.4 1,962,904 - -0.4 1,962,904 - -0.8 1,963,012 - BPHYT_RS08600 0.28 -0.8 1,963,561 + -0.2 1,963,562 - -0.1 1,963,684 + +1.0 1,963,685 - +2.0 1,963,705 + -1.4 1,963,705 + +0.5 1,963,709 + +0.8 1,963,710 - -0.6 1,963,830 + -1.1 1,963,885 + BPHYT_RS08610 0.13 -0.7 1,963,886 - BPHYT_RS08610 0.13 -2.1 1,963,886 - BPHYT_RS08610 0.13 +1.1 1,963,960 + BPHYT_RS08610 0.40 +0.7 1,963,960 + BPHYT_RS08610 0.40 -1.0 1,963,961 - BPHYT_RS08610 0.40 -0.4 1,964,010 + BPHYT_RS08610 0.58 -0.6 1,964,011 - BPHYT_RS08610 0.58 -0.1 1,964,099 - BPHYT_RS08610 0.90 -1.0 1,964,232 - -2.2 1,964,439 + BPHYT_RS08615 0.47 +0.6
Or see this region's nucleotide sequence