Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS07985 and BPHYT_RS07990 are separated by 153 nucleotides BPHYT_RS07990 and BPHYT_RS07995 are separated by 83 nucleotides BPHYT_RS07995 and BPHYT_RS08000 are separated by 553 nucleotides BPHYT_RS08000 and BPHYT_RS08005 are separated by 83 nucleotides
BPHYT_RS07985: BPHYT_RS07985 - membrane protein, at 1,823,780 to 1,824,421
_RS07985
BPHYT_RS07990: BPHYT_RS07990 - peptide chain release factor I, at 1,824,575 to 1,824,979
_RS07990
BPHYT_RS07995: BPHYT_RS07995 - diguanylate phosphodiesterase, at 1,825,063 to 1,826,316
_RS07995
BPHYT_RS08000: BPHYT_RS08000 - antibiotic biosynthesis monooxygenase, at 1,826,870 to 1,827,160
_RS08000
BPHYT_RS08005: BPHYT_RS08005 - DNA-binding protein, at 1,827,244 to 1,827,501
_RS08005
Position (kb)
1825
1826
1827 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1824.090 kb on + strand, within BPHYT_RS07985 at 1824.430 kb on - strand at 1824.482 kb on - strand at 1824.543 kb on + strand at 1824.543 kb on + strand at 1824.544 kb on - strand at 1824.544 kb on - strand at 1824.544 kb on - strand at 1824.587 kb on + strand at 1825.134 kb on - strand at 1825.214 kb on + strand, within BPHYT_RS07995 at 1825.215 kb on - strand, within BPHYT_RS07995 at 1825.250 kb on + strand, within BPHYT_RS07995 at 1825.251 kb on - strand, within BPHYT_RS07995 at 1825.407 kb on - strand, within BPHYT_RS07995 at 1825.407 kb on - strand, within BPHYT_RS07995 at 1825.892 kb on - strand, within BPHYT_RS07995 at 1826.168 kb on + strand, within BPHYT_RS07995 at 1826.218 kb on - strand at 1826.218 kb on - strand at 1826.316 kb on + strand at 1826.432 kb on - strand at 1826.499 kb on - strand at 1826.530 kb on + strand at 1826.681 kb on + strand at 1826.987 kb on + strand, within BPHYT_RS08000 at 1826.987 kb on + strand, within BPHYT_RS08000 at 1827.098 kb on - strand, within BPHYT_RS08000 at 1827.175 kb on + strand at 1827.185 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 1,824,090 + BPHYT_RS07985 0.48 +1.8 1,824,430 - -2.6 1,824,482 - -0.8 1,824,543 + +0.9 1,824,543 + +0.4 1,824,544 - -0.3 1,824,544 - -0.1 1,824,544 - -1.4 1,824,587 + +0.3 1,825,134 - +0.1 1,825,214 + BPHYT_RS07995 0.12 +0.1 1,825,215 - BPHYT_RS07995 0.12 -0.6 1,825,250 + BPHYT_RS07995 0.15 -0.5 1,825,251 - BPHYT_RS07995 0.15 -1.1 1,825,407 - BPHYT_RS07995 0.27 -0.8 1,825,407 - BPHYT_RS07995 0.27 -1.0 1,825,892 - BPHYT_RS07995 0.66 -1.4 1,826,168 + BPHYT_RS07995 0.88 -0.8 1,826,218 - -0.1 1,826,218 - -1.2 1,826,316 + -0.8 1,826,432 - -0.7 1,826,499 - +0.6 1,826,530 + -0.3 1,826,681 + -0.6 1,826,987 + BPHYT_RS08000 0.40 +0.8 1,826,987 + BPHYT_RS08000 0.40 -0.0 1,827,098 - BPHYT_RS08000 0.78 -2.2 1,827,175 + -0.2 1,827,185 + +0.5
Or see this region's nucleotide sequence