Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS07865 and BPHYT_RS07870 overlap by 4 nucleotides BPHYT_RS07870 and BPHYT_RS07875 are separated by 96 nucleotides BPHYT_RS07875 and BPHYT_RS07880 are separated by 535 nucleotides BPHYT_RS07880 and BPHYT_RS07885 are separated by 201 nucleotides
BPHYT_RS07865: BPHYT_RS07865 - NADH oxidase, at 1,791,510 to 1,792,532
_RS07865
BPHYT_RS07870: BPHYT_RS07870 - 1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate dehydrogenase, at 1,792,529 to 1,793,305
_RS07870
BPHYT_RS07875: BPHYT_RS07875 - CoA transferase, at 1,793,402 to 1,795,393
_RS07875
BPHYT_RS07880: BPHYT_RS07880 - hypothetical protein, at 1,795,929 to 1,796,183
_RS07880
BPHYT_RS07885: BPHYT_RS07885 - hypothetical protein, at 1,796,385 to 1,796,669
_RS07885
Position (kb)
1793
1794
1795
1796 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1792.887 kb on - strand, within BPHYT_RS07870 at 1792.986 kb on - strand, within BPHYT_RS07870 at 1793.259 kb on - strand at 1793.443 kb on - strand at 1793.443 kb on - strand at 1793.446 kb on + strand at 1793.449 kb on - strand at 1793.449 kb on - strand at 1793.449 kb on - strand at 1793.449 kb on - strand at 1793.746 kb on - strand, within BPHYT_RS07875 at 1793.964 kb on + strand, within BPHYT_RS07875 at 1793.964 kb on + strand, within BPHYT_RS07875 at 1793.964 kb on + strand, within BPHYT_RS07875 at 1793.965 kb on - strand, within BPHYT_RS07875 at 1793.994 kb on + strand, within BPHYT_RS07875 at 1794.083 kb on - strand, within BPHYT_RS07875 at 1794.264 kb on + strand, within BPHYT_RS07875 at 1794.279 kb on + strand, within BPHYT_RS07875 at 1794.837 kb on + strand, within BPHYT_RS07875 at 1794.838 kb on - strand, within BPHYT_RS07875 at 1794.838 kb on - strand, within BPHYT_RS07875 at 1794.838 kb on - strand, within BPHYT_RS07875 at 1794.937 kb on + strand, within BPHYT_RS07875 at 1794.937 kb on + strand, within BPHYT_RS07875 at 1795.023 kb on + strand, within BPHYT_RS07875 at 1795.023 kb on + strand, within BPHYT_RS07875 at 1795.023 kb on + strand, within BPHYT_RS07875 at 1795.024 kb on - strand, within BPHYT_RS07875 at 1795.438 kb on + strand at 1795.439 kb on - strand at 1795.492 kb on - strand at 1795.549 kb on + strand at 1795.721 kb on + strand at 1795.721 kb on + strand at 1795.722 kb on - strand at 1795.722 kb on - strand at 1795.901 kb on + strand at 1795.928 kb on + strand at 1795.929 kb on - strand at 1795.929 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 1,792,887 - BPHYT_RS07870 0.46 -0.9 1,792,986 - BPHYT_RS07870 0.59 -0.4 1,793,259 - -1.8 1,793,443 - +0.2 1,793,443 - -0.7 1,793,446 + -0.4 1,793,449 - +0.0 1,793,449 - -0.3 1,793,449 - -1.7 1,793,449 - -0.2 1,793,746 - BPHYT_RS07875 0.17 -0.9 1,793,964 + BPHYT_RS07875 0.28 -2.5 1,793,964 + BPHYT_RS07875 0.28 -0.2 1,793,964 + BPHYT_RS07875 0.28 +0.1 1,793,965 - BPHYT_RS07875 0.28 +0.9 1,793,994 + BPHYT_RS07875 0.30 +0.0 1,794,083 - BPHYT_RS07875 0.34 +2.0 1,794,264 + BPHYT_RS07875 0.43 -1.0 1,794,279 + BPHYT_RS07875 0.44 +1.5 1,794,837 + BPHYT_RS07875 0.72 +0.6 1,794,838 - BPHYT_RS07875 0.72 -0.4 1,794,838 - BPHYT_RS07875 0.72 -1.5 1,794,838 - BPHYT_RS07875 0.72 -0.7 1,794,937 + BPHYT_RS07875 0.77 -0.4 1,794,937 + BPHYT_RS07875 0.77 +1.7 1,795,023 + BPHYT_RS07875 0.81 -1.7 1,795,023 + BPHYT_RS07875 0.81 +0.8 1,795,023 + BPHYT_RS07875 0.81 -0.6 1,795,024 - BPHYT_RS07875 0.81 -0.5 1,795,438 + +0.3 1,795,439 - -2.2 1,795,492 - +0.6 1,795,549 + -1.4 1,795,721 + -0.4 1,795,721 + -0.1 1,795,722 - +0.2 1,795,722 - +0.1 1,795,901 + -0.4 1,795,928 + +0.3 1,795,929 - +0.4 1,795,929 - -1.1
Or see this region's nucleotide sequence