Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04205 and BPHYT_RS04210 are separated by 289 nucleotides BPHYT_RS04210 and BPHYT_RS04215 are separated by 255 nucleotides BPHYT_RS04215 and BPHYT_RS04220 are separated by 253 nucleotides BPHYT_RS04220 and BPHYT_RS04225 overlap by 4 nucleotides
BPHYT_RS04205: BPHYT_RS04205 - kinase, at 964,746 to 965,606
_RS04205
BPHYT_RS04210: BPHYT_RS04210 - rubredoxin, at 965,896 to 966,066
_RS04210
BPHYT_RS04215: BPHYT_RS04215 - hypothetical protein, at 966,322 to 967,734
_RS04215
BPHYT_RS04220: BPHYT_RS04220 - hypothetical protein, at 967,988 to 968,566
_RS04220
BPHYT_RS04225: BPHYT_RS04225 - Holliday junction resolvase, at 968,563 to 969,003
_RS04225
Position (kb) 966
967
968 Strain fitness (log2 ratio) -2
-1
0
1 at 965.663 kb on + strand at 965.663 kb on + strand at 965.664 kb on - strand at 965.805 kb on - strand at 965.879 kb on + strand at 966.228 kb on + strand at 966.228 kb on + strand at 966.229 kb on - strand at 966.947 kb on - strand, within BPHYT_RS04215 at 967.190 kb on - strand, within BPHYT_RS04215 at 967.336 kb on + strand, within BPHYT_RS04215 at 967.336 kb on + strand, within BPHYT_RS04215 at 967.820 kb on + strand at 967.820 kb on + strand at 967.820 kb on + strand at 967.820 kb on + strand at 967.820 kb on + strand at 967.821 kb on - strand at 967.821 kb on - strand at 967.821 kb on - strand at 967.876 kb on + strand at 967.877 kb on - strand at 967.888 kb on + strand at 967.888 kb on + strand at 967.987 kb on + strand at 967.987 kb on + strand at 967.987 kb on + strand at 967.987 kb on + strand at 967.988 kb on - strand at 968.081 kb on + strand, within BPHYT_RS04220 at 968.213 kb on + strand, within BPHYT_RS04220 at 968.213 kb on + strand, within BPHYT_RS04220 at 968.279 kb on + strand, within BPHYT_RS04220
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 965,663 + +0.4 965,663 + +0.0 965,664 - -0.3 965,805 - +1.2 965,879 + +0.0 966,228 + +0.3 966,228 + -0.2 966,229 - -0.8 966,947 - BPHYT_RS04215 0.44 -1.3 967,190 - BPHYT_RS04215 0.61 -0.1 967,336 + BPHYT_RS04215 0.72 -0.4 967,336 + BPHYT_RS04215 0.72 -0.9 967,820 + -0.4 967,820 + -0.7 967,820 + +0.0 967,820 + -1.9 967,820 + -1.5 967,821 - +0.2 967,821 - -0.4 967,821 - -0.7 967,876 + -2.1 967,877 - +1.4 967,888 + +0.5 967,888 + -0.1 967,987 + -0.8 967,987 + +1.1 967,987 + +0.2 967,987 + +0.0 967,988 - +0.8 968,081 + BPHYT_RS04220 0.16 -0.9 968,213 + BPHYT_RS04220 0.39 -1.4 968,213 + BPHYT_RS04220 0.39 -0.6 968,279 + BPHYT_RS04220 0.50 -0.2
Or see this region's nucleotide sequence