Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04185 and BPHYT_RS04190 are separated by 95 nucleotides BPHYT_RS04190 and BPHYT_RS04195 are separated by 416 nucleotides BPHYT_RS04195 and BPHYT_RS04200 are separated by 55 nucleotides
BPHYT_RS04185: BPHYT_RS04185 - diguanylate cyclase, at 959,872 to 961,116
_RS04185
BPHYT_RS04190: BPHYT_RS04190 - AraC family transcriptional regulator, at 961,212 to 962,168
_RS04190
BPHYT_RS04195: BPHYT_RS04195 - molecular chaperone GroES, at 962,585 to 962,875
_RS04195
BPHYT_RS04200: BPHYT_RS04200 - molecular chaperone GroEL, at 962,931 to 964,571
_RS04200
Position (kb) 961
962
963 Strain fitness (log2 ratio) -2
-1
0
1
2 at 960.276 kb on + strand, within BPHYT_RS04185 at 960.277 kb on - strand, within BPHYT_RS04185 at 960.278 kb on + strand, within BPHYT_RS04185 at 960.278 kb on + strand, within BPHYT_RS04185 at 960.278 kb on + strand, within BPHYT_RS04185 at 960.278 kb on + strand, within BPHYT_RS04185 at 960.278 kb on + strand, within BPHYT_RS04185 at 960.279 kb on - strand, within BPHYT_RS04185 at 960.380 kb on - strand, within BPHYT_RS04185 at 960.438 kb on - strand, within BPHYT_RS04185 at 960.470 kb on + strand, within BPHYT_RS04185 at 960.703 kb on - strand, within BPHYT_RS04185 at 960.703 kb on - strand, within BPHYT_RS04185 at 960.703 kb on - strand, within BPHYT_RS04185 at 960.703 kb on - strand, within BPHYT_RS04185 at 960.710 kb on + strand, within BPHYT_RS04185 at 960.710 kb on + strand, within BPHYT_RS04185 at 960.710 kb on + strand, within BPHYT_RS04185 at 960.711 kb on - strand, within BPHYT_RS04185 at 960.756 kb on - strand, within BPHYT_RS04185 at 961.040 kb on + strand at 961.040 kb on + strand at 961.041 kb on - strand at 961.224 kb on + strand at 961.224 kb on + strand at 961.224 kb on + strand at 961.225 kb on - strand at 961.225 kb on - strand at 961.267 kb on - strand at 961.356 kb on + strand, within BPHYT_RS04190 at 961.380 kb on + strand, within BPHYT_RS04190 at 961.381 kb on - strand, within BPHYT_RS04190 at 961.385 kb on - strand, within BPHYT_RS04190 at 961.672 kb on + strand, within BPHYT_RS04190 at 961.766 kb on + strand, within BPHYT_RS04190 at 961.766 kb on + strand, within BPHYT_RS04190 at 961.766 kb on + strand, within BPHYT_RS04190 at 961.767 kb on - strand, within BPHYT_RS04190 at 961.905 kb on + strand, within BPHYT_RS04190 at 961.905 kb on + strand, within BPHYT_RS04190 at 961.906 kb on - strand, within BPHYT_RS04190 at 961.906 kb on - strand, within BPHYT_RS04190 at 961.907 kb on + strand, within BPHYT_RS04190 at 962.167 kb on - strand at 962.281 kb on - strand at 962.327 kb on - strand at 962.327 kb on - strand at 962.394 kb on + strand at 962.394 kb on + strand at 962.394 kb on + strand at 962.395 kb on - strand at 962.395 kb on - strand at 962.403 kb on + strand at 962.434 kb on + strand at 962.434 kb on + strand at 962.434 kb on + strand at 962.434 kb on + strand at 962.435 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 960,276 + BPHYT_RS04185 0.32 +0.5 960,277 - BPHYT_RS04185 0.33 -0.2 960,278 + BPHYT_RS04185 0.33 +0.3 960,278 + BPHYT_RS04185 0.33 -2.0 960,278 + BPHYT_RS04185 0.33 +2.0 960,278 + BPHYT_RS04185 0.33 +0.2 960,278 + BPHYT_RS04185 0.33 -0.3 960,279 - BPHYT_RS04185 0.33 -0.6 960,380 - BPHYT_RS04185 0.41 -0.6 960,438 - BPHYT_RS04185 0.45 +0.4 960,470 + BPHYT_RS04185 0.48 -0.1 960,703 - BPHYT_RS04185 0.67 -0.5 960,703 - BPHYT_RS04185 0.67 -0.4 960,703 - BPHYT_RS04185 0.67 +0.0 960,703 - BPHYT_RS04185 0.67 +0.4 960,710 + BPHYT_RS04185 0.67 +0.1 960,710 + BPHYT_RS04185 0.67 +1.0 960,710 + BPHYT_RS04185 0.67 +0.2 960,711 - BPHYT_RS04185 0.67 -1.4 960,756 - BPHYT_RS04185 0.71 -0.2 961,040 + -1.5 961,040 + -0.8 961,041 - -0.3 961,224 + +0.4 961,224 + +0.4 961,224 + -0.7 961,225 - +0.7 961,225 - -1.1 961,267 - -1.5 961,356 + BPHYT_RS04190 0.15 -0.3 961,380 + BPHYT_RS04190 0.18 +0.8 961,381 - BPHYT_RS04190 0.18 +0.2 961,385 - BPHYT_RS04190 0.18 -0.1 961,672 + BPHYT_RS04190 0.48 +1.1 961,766 + BPHYT_RS04190 0.58 +0.0 961,766 + BPHYT_RS04190 0.58 -0.2 961,766 + BPHYT_RS04190 0.58 +0.9 961,767 - BPHYT_RS04190 0.58 -0.0 961,905 + BPHYT_RS04190 0.72 -0.9 961,905 + BPHYT_RS04190 0.72 -0.7 961,906 - BPHYT_RS04190 0.73 +0.5 961,906 - BPHYT_RS04190 0.73 -1.0 961,907 + BPHYT_RS04190 0.73 +0.5 962,167 - +0.3 962,281 - -0.5 962,327 - +0.5 962,327 - +0.2 962,394 + -0.7 962,394 + -1.0 962,394 + -1.2 962,395 - -0.3 962,395 - -0.8 962,403 + -1.8 962,434 + -0.6 962,434 + -0.6 962,434 + -1.2 962,434 + -0.6 962,435 - -0.1
Or see this region's nucleotide sequence