Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03605 and BPHYT_RS03610 are separated by 39 nucleotides BPHYT_RS03610 and BPHYT_RS03615 overlap by 4 nucleotides BPHYT_RS03615 and BPHYT_RS03620 are separated by 48 nucleotides
BPHYT_RS03605: BPHYT_RS03605 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 820,505 to 821,320
_RS03605
BPHYT_RS03610: BPHYT_RS03610 - deoxyadenosine kinase, at 821,360 to 822,040
_RS03610
BPHYT_RS03615: BPHYT_RS03615 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase, at 822,037 to 822,585
_RS03615
BPHYT_RS03620: BPHYT_RS03620 - poly(A) polymerase, at 822,634 to 824,223
_RS03620
Position (kb) 821
822
823 Strain fitness (log2 ratio) -1
0
1 at 820.417 kb on - strand at 820.417 kb on - strand at 820.417 kb on - strand at 820.514 kb on - strand at 821.484 kb on - strand, within BPHYT_RS03610 at 821.500 kb on - strand, within BPHYT_RS03610 at 821.506 kb on - strand, within BPHYT_RS03610 at 821.521 kb on - strand, within BPHYT_RS03610 at 821.545 kb on - strand, within BPHYT_RS03610 at 821.834 kb on - strand, within BPHYT_RS03610 at 822.089 kb on - strand at 822.089 kb on - strand at 822.631 kb on - strand at 822.631 kb on - strand at 822.631 kb on - strand at 822.818 kb on - strand, within BPHYT_RS03620 at 822.818 kb on - strand, within BPHYT_RS03620 at 822.818 kb on - strand, within BPHYT_RS03620 at 822.818 kb on - strand, within BPHYT_RS03620 at 822.890 kb on + strand, within BPHYT_RS03620
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 820,417 - -0.5 820,417 - -0.4 820,417 - +1.5 820,514 - -0.9 821,484 - BPHYT_RS03610 0.18 -0.8 821,500 - BPHYT_RS03610 0.21 -0.5 821,506 - BPHYT_RS03610 0.21 -1.4 821,521 - BPHYT_RS03610 0.24 +0.9 821,545 - BPHYT_RS03610 0.27 -0.7 821,834 - BPHYT_RS03610 0.70 -0.9 822,089 - +0.9 822,089 - -0.9 822,631 - +0.3 822,631 - +1.2 822,631 - +0.3 822,818 - BPHYT_RS03620 0.12 -0.1 822,818 - BPHYT_RS03620 0.12 -0.5 822,818 - BPHYT_RS03620 0.12 -0.0 822,818 - BPHYT_RS03620 0.12 -0.5 822,890 + BPHYT_RS03620 0.16 +1.1
Or see this region's nucleotide sequence