Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03230 and BPHYT_RS03235 are separated by 118 nucleotides BPHYT_RS03235 and BPHYT_RS03240 are separated by 140 nucleotides
BPHYT_RS03230: BPHYT_RS03230 - acetyl-CoA acetyltransferase, at 726,094 to 727,293
_RS03230
BPHYT_RS03235: BPHYT_RS03235 - enoyl-CoA hydratase, at 727,412 to 728,182
_RS03235
BPHYT_RS03240: BPHYT_RS03240 - formate dehydrogenase accessory protein FdhD, at 728,323 to 729,162
_RS03240
Position (kb) 727
728
729 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 726.492 kb on + strand, within BPHYT_RS03230 at 726.492 kb on + strand, within BPHYT_RS03230 at 726.493 kb on - strand, within BPHYT_RS03230 at 726.513 kb on + strand, within BPHYT_RS03230 at 726.548 kb on - strand, within BPHYT_RS03230 at 726.849 kb on + strand, within BPHYT_RS03230 at 726.850 kb on - strand, within BPHYT_RS03230 at 726.850 kb on - strand, within BPHYT_RS03230 at 726.850 kb on - strand, within BPHYT_RS03230 at 727.228 kb on + strand at 727.228 kb on + strand at 727.229 kb on - strand at 727.261 kb on - strand at 727.423 kb on + strand at 727.493 kb on - strand, within BPHYT_RS03235 at 727.505 kb on + strand, within BPHYT_RS03235 at 727.505 kb on + strand, within BPHYT_RS03235 at 728.205 kb on + strand at 728.346 kb on + strand at 728.347 kb on - strand at 728.360 kb on + strand at 728.361 kb on - strand at 728.493 kb on - strand, within BPHYT_RS03240 at 728.493 kb on - strand, within BPHYT_RS03240 at 729.165 kb on + strand at 729.166 kb on - strand at 729.166 kb on - strand at 729.166 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 726,492 + BPHYT_RS03230 0.33 +0.1 726,492 + BPHYT_RS03230 0.33 -0.7 726,493 - BPHYT_RS03230 0.33 +0.3 726,513 + BPHYT_RS03230 0.35 -2.0 726,548 - BPHYT_RS03230 0.38 -0.5 726,849 + BPHYT_RS03230 0.63 -0.2 726,850 - BPHYT_RS03230 0.63 +0.4 726,850 - BPHYT_RS03230 0.63 +0.1 726,850 - BPHYT_RS03230 0.63 +0.0 727,228 + +0.1 727,228 + -0.4 727,229 - -0.3 727,261 - -1.3 727,423 + -1.5 727,493 - BPHYT_RS03235 0.11 -0.0 727,505 + BPHYT_RS03235 0.12 -3.0 727,505 + BPHYT_RS03235 0.12 -1.6 728,205 + +0.1 728,346 + -0.8 728,347 - +0.3 728,360 + +0.5 728,361 - +0.1 728,493 - BPHYT_RS03240 0.20 -0.2 728,493 - BPHYT_RS03240 0.20 -0.1 729,165 + -0.1 729,166 - -0.8 729,166 - +0.1 729,166 - -0.7
Or see this region's nucleotide sequence