Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02325 and BPHYT_RS02330 are separated by 281 nucleotides BPHYT_RS02330 and BPHYT_RS02335 are separated by 128 nucleotides
BPHYT_RS02325: BPHYT_RS02325 - hypothetical protein, at 532,651 to 533,958
_RS02325
BPHYT_RS02330: BPHYT_RS02330 - IclR family transcriptional regulator, at 534,240 to 535,142
_RS02330
BPHYT_RS02335: BPHYT_RS02335 - fumarylacetoacetate hydrolase, at 535,271 to 536,254
_RS02335
Position (kb) 534
535
536 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 533.322 kb on - strand, within BPHYT_RS02325 at 533.369 kb on + strand, within BPHYT_RS02325 at 533.370 kb on - strand, within BPHYT_RS02325 at 533.427 kb on - strand, within BPHYT_RS02325 at 533.427 kb on - strand, within BPHYT_RS02325 at 533.745 kb on + strand, within BPHYT_RS02325 at 533.746 kb on - strand, within BPHYT_RS02325 at 533.746 kb on - strand, within BPHYT_RS02325 at 534.048 kb on + strand at 534.049 kb on - strand at 534.065 kb on - strand at 534.263 kb on + strand at 534.264 kb on - strand at 534.264 kb on - strand at 534.264 kb on - strand at 534.265 kb on + strand at 534.266 kb on - strand at 534.277 kb on + strand at 534.277 kb on + strand at 534.278 kb on - strand at 534.335 kb on + strand, within BPHYT_RS02330 at 534.586 kb on + strand, within BPHYT_RS02330 at 534.587 kb on - strand, within BPHYT_RS02330 at 534.895 kb on + strand, within BPHYT_RS02330 at 534.896 kb on - strand, within BPHYT_RS02330 at 535.178 kb on + strand at 535.178 kb on + strand at 535.179 kb on - strand at 535.179 kb on - strand at 535.179 kb on - strand at 535.227 kb on + strand at 535.227 kb on + strand at 535.228 kb on - strand at 535.233 kb on - strand at 535.233 kb on - strand at 535.252 kb on + strand at 535.532 kb on + strand, within BPHYT_RS02335 at 535.532 kb on + strand, within BPHYT_RS02335 at 535.532 kb on + strand, within BPHYT_RS02335 at 535.554 kb on + strand, within BPHYT_RS02335 at 535.653 kb on + strand, within BPHYT_RS02335 at 535.653 kb on + strand, within BPHYT_RS02335 at 535.653 kb on + strand, within BPHYT_RS02335 at 535.653 kb on + strand, within BPHYT_RS02335 at 535.654 kb on - strand, within BPHYT_RS02335 at 535.654 kb on - strand, within BPHYT_RS02335 at 535.654 kb on - strand, within BPHYT_RS02335 at 536.094 kb on - strand, within BPHYT_RS02335
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 533,322 - BPHYT_RS02325 0.51 +0.5 533,369 + BPHYT_RS02325 0.55 -0.7 533,370 - BPHYT_RS02325 0.55 +0.3 533,427 - BPHYT_RS02325 0.59 -1.7 533,427 - BPHYT_RS02325 0.59 +0.4 533,745 + BPHYT_RS02325 0.84 +0.8 533,746 - BPHYT_RS02325 0.84 +1.1 533,746 - BPHYT_RS02325 0.84 +0.0 534,048 + +4.5 534,049 - -1.5 534,065 - +0.4 534,263 + -0.0 534,264 - -0.0 534,264 - +0.2 534,264 - -0.6 534,265 + +0.9 534,266 - -0.8 534,277 + -1.1 534,277 + +0.1 534,278 - +0.3 534,335 + BPHYT_RS02330 0.11 -0.0 534,586 + BPHYT_RS02330 0.38 -0.5 534,587 - BPHYT_RS02330 0.38 +1.3 534,895 + BPHYT_RS02330 0.73 +1.0 534,896 - BPHYT_RS02330 0.73 +1.1 535,178 + -0.7 535,178 + -0.2 535,179 - -1.6 535,179 - -0.7 535,179 - -0.4 535,227 + +1.0 535,227 + -0.2 535,228 - +0.6 535,233 - -0.0 535,233 - +0.1 535,252 + +0.1 535,532 + BPHYT_RS02335 0.27 +0.2 535,532 + BPHYT_RS02335 0.27 -0.2 535,532 + BPHYT_RS02335 0.27 -1.2 535,554 + BPHYT_RS02335 0.29 -0.4 535,653 + BPHYT_RS02335 0.39 +0.5 535,653 + BPHYT_RS02335 0.39 -0.5 535,653 + BPHYT_RS02335 0.39 -0.3 535,653 + BPHYT_RS02335 0.39 -0.4 535,654 - BPHYT_RS02335 0.39 -0.2 535,654 - BPHYT_RS02335 0.39 -1.8 535,654 - BPHYT_RS02335 0.39 -2.9 536,094 - BPHYT_RS02335 0.84 -0.1
Or see this region's nucleotide sequence