Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02115 and BPHYT_RS02120 overlap by 4 nucleotides BPHYT_RS02120 and BPHYT_RS02125 are separated by 19 nucleotides BPHYT_RS02125 and BPHYT_RS02130 are separated by 54 nucleotides BPHYT_RS02130 and BPHYT_RS02135 are separated by 60 nucleotides
BPHYT_RS02115: BPHYT_RS02115 - acyl dehydratase, at 486,833 to 487,324
_RS02115
BPHYT_RS02120: BPHYT_RS02120 - hypothetical protein, at 487,321 to 487,545
_RS02120
BPHYT_RS02125: BPHYT_RS02125 - prolyl-tRNA synthetase, at 487,565 to 488,086
_RS02125
BPHYT_RS02130: BPHYT_RS02130 - hydroxymethylglutaryl-CoA lyase, at 488,141 to 489,067
_RS02130
BPHYT_RS02135: BPHYT_RS02135 - glyoxylate reductase, at 489,128 to 490,069
_RS02135
Position (kb) 488
489
490 Strain fitness (log2 ratio) -2
-1
0
1 at 487.440 kb on + strand, within BPHYT_RS02120 at 487.441 kb on - strand, within BPHYT_RS02120 at 488.299 kb on - strand, within BPHYT_RS02130 at 488.299 kb on - strand, within BPHYT_RS02130 at 489.079 kb on + strand at 489.080 kb on - strand at 489.124 kb on - strand at 489.405 kb on + strand, within BPHYT_RS02135 at 489.405 kb on + strand, within BPHYT_RS02135 at 489.763 kb on - strand, within BPHYT_RS02135 at 489.818 kb on - strand, within BPHYT_RS02135
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 487,440 + BPHYT_RS02120 0.53 -2.2 487,441 - BPHYT_RS02120 0.53 +0.4 488,299 - BPHYT_RS02130 0.17 -1.3 488,299 - BPHYT_RS02130 0.17 -1.1 489,079 + +0.7 489,080 - -0.1 489,124 - +0.1 489,405 + BPHYT_RS02135 0.29 -0.3 489,405 + BPHYT_RS02135 0.29 +1.6 489,763 - BPHYT_RS02135 0.67 +0.6 489,818 - BPHYT_RS02135 0.73 +0.4
Or see this region's nucleotide sequence