Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01695 and BPHYT_RS01700 are separated by 65 nucleotides BPHYT_RS01700 and BPHYT_RS01705 are separated by 272 nucleotides BPHYT_RS01705 and BPHYT_RS01710 are separated by 241 nucleotides
BPHYT_RS01695: BPHYT_RS01695 - L-glyceraldehyde 3-phosphate reductase, at 383,542 to 384,585
_RS01695
BPHYT_RS01700: BPHYT_RS01700 - membrane protein, at 384,651 to 384,836
_RS01700
BPHYT_RS01705: BPHYT_RS01705 - MFS transporter, at 385,109 to 386,311
_RS01705
BPHYT_RS01710: BPHYT_RS01710 - RND transporter, at 386,553 to 388,082
_RS01710
Position (kb) 385
386
387 Strain fitness (log2 ratio) -2
-1
0
1 at 384.130 kb on + strand, within BPHYT_RS01695 at 384.241 kb on + strand, within BPHYT_RS01695 at 384.242 kb on - strand, within BPHYT_RS01695 at 384.826 kb on + strand at 384.826 kb on + strand at 384.848 kb on + strand at 384.848 kb on + strand at 384.849 kb on - strand at 384.849 kb on - strand at 384.879 kb on + strand at 384.880 kb on - strand at 384.914 kb on - strand at 384.915 kb on + strand at 384.916 kb on - strand at 384.916 kb on - strand at 384.936 kb on - strand at 384.936 kb on - strand at 384.937 kb on + strand at 385.053 kb on + strand at 385.054 kb on - strand at 385.231 kb on - strand, within BPHYT_RS01705 at 385.257 kb on + strand, within BPHYT_RS01705 at 385.258 kb on - strand, within BPHYT_RS01705 at 385.260 kb on + strand, within BPHYT_RS01705 at 385.272 kb on + strand, within BPHYT_RS01705 at 385.519 kb on - strand, within BPHYT_RS01705 at 385.519 kb on - strand, within BPHYT_RS01705 at 385.519 kb on - strand, within BPHYT_RS01705 at 385.519 kb on - strand, within BPHYT_RS01705 at 385.560 kb on + strand, within BPHYT_RS01705 at 385.560 kb on + strand, within BPHYT_RS01705 at 385.561 kb on - strand, within BPHYT_RS01705 at 385.561 kb on - strand, within BPHYT_RS01705 at 385.590 kb on + strand, within BPHYT_RS01705 at 385.590 kb on + strand, within BPHYT_RS01705 at 385.590 kb on + strand, within BPHYT_RS01705 at 385.596 kb on + strand, within BPHYT_RS01705 at 385.597 kb on - strand, within BPHYT_RS01705 at 385.929 kb on + strand, within BPHYT_RS01705 at 385.929 kb on + strand, within BPHYT_RS01705 at 385.929 kb on + strand, within BPHYT_RS01705 at 385.930 kb on - strand, within BPHYT_RS01705 at 386.048 kb on - strand, within BPHYT_RS01705 at 386.048 kb on - strand, within BPHYT_RS01705 at 386.215 kb on - strand at 386.356 kb on + strand at 386.357 kb on - strand at 386.448 kb on + strand at 386.448 kb on + strand at 386.555 kb on - strand at 386.769 kb on - strand, within BPHYT_RS01710 at 387.043 kb on + strand, within BPHYT_RS01710 at 387.043 kb on + strand, within BPHYT_RS01710 at 387.043 kb on + strand, within BPHYT_RS01710 at 387.043 kb on + strand, within BPHYT_RS01710 at 387.044 kb on - strand, within BPHYT_RS01710 at 387.044 kb on - strand, within BPHYT_RS01710
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 384,130 + BPHYT_RS01695 0.56 -1.0 384,241 + BPHYT_RS01695 0.67 -1.3 384,242 - BPHYT_RS01695 0.67 -0.8 384,826 + +0.2 384,826 + +0.3 384,848 + -0.4 384,848 + -0.1 384,849 - +1.3 384,849 - -0.3 384,879 + -1.6 384,880 - +0.4 384,914 - +0.7 384,915 + -1.1 384,916 - +0.4 384,916 - -0.1 384,936 - +0.5 384,936 - -0.2 384,937 + -1.0 385,053 + +1.6 385,054 - +0.6 385,231 - BPHYT_RS01705 0.10 -1.2 385,257 + BPHYT_RS01705 0.12 -0.9 385,258 - BPHYT_RS01705 0.12 -0.9 385,260 + BPHYT_RS01705 0.13 +0.2 385,272 + BPHYT_RS01705 0.14 -1.3 385,519 - BPHYT_RS01705 0.34 +0.2 385,519 - BPHYT_RS01705 0.34 -0.8 385,519 - BPHYT_RS01705 0.34 +0.2 385,519 - BPHYT_RS01705 0.34 +0.4 385,560 + BPHYT_RS01705 0.37 +0.8 385,560 + BPHYT_RS01705 0.37 +0.4 385,561 - BPHYT_RS01705 0.38 +0.1 385,561 - BPHYT_RS01705 0.38 -0.5 385,590 + BPHYT_RS01705 0.40 -1.1 385,590 + BPHYT_RS01705 0.40 +0.6 385,590 + BPHYT_RS01705 0.40 -0.5 385,596 + BPHYT_RS01705 0.40 -0.9 385,597 - BPHYT_RS01705 0.41 -0.6 385,929 + BPHYT_RS01705 0.68 -0.1 385,929 + BPHYT_RS01705 0.68 +0.1 385,929 + BPHYT_RS01705 0.68 +1.0 385,930 - BPHYT_RS01705 0.68 -0.8 386,048 - BPHYT_RS01705 0.78 -0.8 386,048 - BPHYT_RS01705 0.78 -1.0 386,215 - -0.7 386,356 + -0.5 386,357 - -0.3 386,448 + +0.0 386,448 + -1.9 386,555 - +0.7 386,769 - BPHYT_RS01710 0.14 -0.9 387,043 + BPHYT_RS01710 0.32 -0.5 387,043 + BPHYT_RS01710 0.32 +1.0 387,043 + BPHYT_RS01710 0.32 +0.2 387,043 + BPHYT_RS01710 0.32 -0.4 387,044 - BPHYT_RS01710 0.32 +0.1 387,044 - BPHYT_RS01710 0.32 -0.7
Or see this region's nucleotide sequence