Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01240 and BPHYT_RS01245 are separated by 95 nucleotides BPHYT_RS01245 and BPHYT_RS01250 are separated by 54 nucleotides BPHYT_RS01250 and BPHYT_RS01255 are separated by 285 nucleotides BPHYT_RS01255 and BPHYT_RS01260 are separated by 215 nucleotides
BPHYT_RS01240: BPHYT_RS01240 - hypothetical protein, at 285,205 to 285,423
_RS01240
BPHYT_RS01245: BPHYT_RS01245 - hypothetical protein, at 285,519 to 286,262
_RS01245
BPHYT_RS01250: BPHYT_RS01250 - integrase, at 286,317 to 287,315
_RS01250
BPHYT_RS01255: BPHYT_RS01255 - AsnC family transcriptional regulator, at 287,601 to 288,062
_RS01255
BPHYT_RS01260: BPHYT_RS01260 - hypothetical protein, at 288,278 to 289,408
_RS01260
Position (kb) 286
287
288 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 285.381 kb on - strand, within BPHYT_RS01240 at 285.427 kb on - strand at 285.527 kb on + strand at 285.591 kb on + strand at 285.693 kb on + strand, within BPHYT_RS01245 at 285.694 kb on - strand, within BPHYT_RS01245 at 285.951 kb on + strand, within BPHYT_RS01245 at 285.951 kb on + strand, within BPHYT_RS01245 at 285.952 kb on - strand, within BPHYT_RS01245 at 285.952 kb on - strand, within BPHYT_RS01245 at 286.058 kb on - strand, within BPHYT_RS01245 at 286.058 kb on - strand, within BPHYT_RS01245 at 286.171 kb on + strand, within BPHYT_RS01245 at 286.172 kb on - strand, within BPHYT_RS01245 at 286.260 kb on + strand at 286.516 kb on + strand, within BPHYT_RS01250 at 286.516 kb on + strand, within BPHYT_RS01250 at 286.522 kb on - strand, within BPHYT_RS01250 at 286.701 kb on + strand, within BPHYT_RS01250 at 286.702 kb on - strand, within BPHYT_RS01250 at 286.934 kb on + strand, within BPHYT_RS01250 at 286.935 kb on - strand, within BPHYT_RS01250 at 287.196 kb on - strand, within BPHYT_RS01250 at 287.228 kb on + strand at 287.228 kb on + strand at 287.229 kb on - strand at 287.229 kb on - strand at 287.533 kb on - strand at 287.652 kb on - strand, within BPHYT_RS01255 at 287.717 kb on - strand, within BPHYT_RS01255 at 287.717 kb on - strand, within BPHYT_RS01255 at 287.756 kb on - strand, within BPHYT_RS01255 at 287.852 kb on - strand, within BPHYT_RS01255 at 287.973 kb on + strand, within BPHYT_RS01255 at 288.074 kb on + strand at 288.075 kb on - strand at 288.142 kb on - strand at 288.142 kb on - strand at 288.175 kb on + strand at 288.301 kb on + strand at 288.301 kb on + strand at 288.301 kb on + strand at 288.302 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 285,381 - BPHYT_RS01240 0.80 -1.3 285,427 - -0.8 285,527 + -0.4 285,591 + +0.0 285,693 + BPHYT_RS01245 0.23 -0.1 285,694 - BPHYT_RS01245 0.24 +2.8 285,951 + BPHYT_RS01245 0.58 -0.6 285,951 + BPHYT_RS01245 0.58 -0.4 285,952 - BPHYT_RS01245 0.58 +0.7 285,952 - BPHYT_RS01245 0.58 -0.4 286,058 - BPHYT_RS01245 0.72 -1.7 286,058 - BPHYT_RS01245 0.72 +1.5 286,171 + BPHYT_RS01245 0.88 -1.4 286,172 - BPHYT_RS01245 0.88 -0.0 286,260 + +0.2 286,516 + BPHYT_RS01250 0.20 +0.5 286,516 + BPHYT_RS01250 0.20 -0.8 286,522 - BPHYT_RS01250 0.21 +0.6 286,701 + BPHYT_RS01250 0.38 +0.3 286,702 - BPHYT_RS01250 0.39 +0.1 286,934 + BPHYT_RS01250 0.62 -0.0 286,935 - BPHYT_RS01250 0.62 +0.4 287,196 - BPHYT_RS01250 0.88 +0.4 287,228 + +0.3 287,228 + +1.1 287,229 - -0.3 287,229 - +0.2 287,533 - -1.0 287,652 - BPHYT_RS01255 0.11 -1.3 287,717 - BPHYT_RS01255 0.25 +1.5 287,717 - BPHYT_RS01255 0.25 -0.8 287,756 - BPHYT_RS01255 0.34 -0.5 287,852 - BPHYT_RS01255 0.54 -0.1 287,973 + BPHYT_RS01255 0.81 -0.0 288,074 + -0.5 288,075 - -0.6 288,142 - +0.2 288,142 - -0.9 288,175 + -0.1 288,301 + +0.8 288,301 + +0.3 288,301 + -1.9 288,302 - -0.4
Or see this region's nucleotide sequence