Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01230 and BPHYT_RS01235 are separated by 461 nucleotides BPHYT_RS01235 and BPHYT_RS01240 are separated by 196 nucleotides BPHYT_RS01240 and BPHYT_RS01245 are separated by 95 nucleotides BPHYT_RS01245 and BPHYT_RS01250 are separated by 54 nucleotides
BPHYT_RS01230: BPHYT_RS01230 - DNA-binding protein, at 284,011 to 284,307
_RS01230
BPHYT_RS01235: BPHYT_RS01235 - hypothetical protein, at 284,769 to 285,008
_RS01235
BPHYT_RS01240: BPHYT_RS01240 - hypothetical protein, at 285,205 to 285,423
_RS01240
BPHYT_RS01245: BPHYT_RS01245 - hypothetical protein, at 285,519 to 286,262
_RS01245
BPHYT_RS01250: BPHYT_RS01250 - integrase, at 286,317 to 287,315
_RS01250
Position (kb) 285
286 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 284.227 kb on - strand, within BPHYT_RS01230 at 284.368 kb on + strand at 284.369 kb on - strand at 284.434 kb on + strand at 284.808 kb on + strand, within BPHYT_RS01235 at 284.809 kb on - strand, within BPHYT_RS01235 at 285.304 kb on - strand, within BPHYT_RS01240 at 285.381 kb on - strand, within BPHYT_RS01240 at 285.427 kb on - strand at 285.527 kb on + strand at 285.591 kb on + strand at 285.693 kb on + strand, within BPHYT_RS01245 at 285.694 kb on - strand, within BPHYT_RS01245 at 285.951 kb on + strand, within BPHYT_RS01245 at 285.951 kb on + strand, within BPHYT_RS01245 at 285.952 kb on - strand, within BPHYT_RS01245 at 285.952 kb on - strand, within BPHYT_RS01245 at 286.058 kb on - strand, within BPHYT_RS01245 at 286.058 kb on - strand, within BPHYT_RS01245 at 286.171 kb on + strand, within BPHYT_RS01245 at 286.172 kb on - strand, within BPHYT_RS01245 at 286.260 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 284,227 - BPHYT_RS01230 0.73 -1.0 284,368 + -0.2 284,369 - +0.3 284,434 + -2.0 284,808 + BPHYT_RS01235 0.16 -0.4 284,809 - BPHYT_RS01235 0.17 -0.4 285,304 - BPHYT_RS01240 0.45 -1.4 285,381 - BPHYT_RS01240 0.80 -1.3 285,427 - -0.8 285,527 + -0.4 285,591 + +0.0 285,693 + BPHYT_RS01245 0.23 -0.1 285,694 - BPHYT_RS01245 0.24 +2.8 285,951 + BPHYT_RS01245 0.58 -0.6 285,951 + BPHYT_RS01245 0.58 -0.4 285,952 - BPHYT_RS01245 0.58 +0.7 285,952 - BPHYT_RS01245 0.58 -0.4 286,058 - BPHYT_RS01245 0.72 -1.7 286,058 - BPHYT_RS01245 0.72 +1.5 286,171 + BPHYT_RS01245 0.88 -1.4 286,172 - BPHYT_RS01245 0.88 -0.0 286,260 + +0.2
Or see this region's nucleotide sequence