Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00365 and BPHYT_RS00370 are separated by 26 nucleotides BPHYT_RS00370 and BPHYT_RS00375 overlap by 4 nucleotides BPHYT_RS00375 and BPHYT_RS00380 are separated by 134 nucleotides BPHYT_RS00380 and BPHYT_RS00385 overlap by 4 nucleotides
BPHYT_RS00365: BPHYT_RS00365 - ABC transporter substrate-binding protein, at 92,781 to 93,749
_RS00365
BPHYT_RS00370: BPHYT_RS00370 - ABC transporter ATP-binding protein, at 93,776 to 94,582
_RS00370
BPHYT_RS00375: BPHYT_RS00375 - sugar ABC transporter, at 94,579 to 95,499
_RS00375
BPHYT_RS00380: BPHYT_RS00380 - 3-octaprenyl-4-hydroxybenzoate carboxy-lyase, at 95,634 to 96,227
_RS00380
BPHYT_RS00385: BPHYT_RS00385 - decarboxylase, at 96,224 to 97,567
_RS00385
Position (kb) 94
95
96 Strain fitness (log2 ratio) -2
-1
0
1
2 at 93.660 kb on + strand at 93.661 kb on - strand at 93.910 kb on - strand, within BPHYT_RS00370 at 93.968 kb on - strand, within BPHYT_RS00370 at 93.968 kb on - strand, within BPHYT_RS00370 at 94.009 kb on + strand, within BPHYT_RS00370 at 94.041 kb on - strand, within BPHYT_RS00370 at 94.442 kb on + strand, within BPHYT_RS00370 at 94.681 kb on - strand, within BPHYT_RS00375 at 94.874 kb on + strand, within BPHYT_RS00375 at 95.133 kb on + strand, within BPHYT_RS00375 at 95.221 kb on - strand, within BPHYT_RS00375 at 95.397 kb on + strand, within BPHYT_RS00375 at 95.398 kb on - strand, within BPHYT_RS00375 at 95.405 kb on + strand, within BPHYT_RS00375 at 95.406 kb on - strand, within BPHYT_RS00375 at 95.504 kb on - strand at 95.704 kb on - strand, within BPHYT_RS00380 at 95.965 kb on - strand, within BPHYT_RS00380 at 95.965 kb on - strand, within BPHYT_RS00380 at 96.164 kb on - strand, within BPHYT_RS00380 at 96.382 kb on - strand, within BPHYT_RS00385 at 96.382 kb on - strand, within BPHYT_RS00385 at 96.419 kb on - strand, within BPHYT_RS00385 at 96.468 kb on + strand, within BPHYT_RS00385 at 96.468 kb on + strand, within BPHYT_RS00385 at 96.468 kb on + strand, within BPHYT_RS00385 at 96.469 kb on - strand, within BPHYT_RS00385 at 96.469 kb on - strand, within BPHYT_RS00385
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 93,660 + -0.2 93,661 - -1.1 93,910 - BPHYT_RS00370 0.17 +1.7 93,968 - BPHYT_RS00370 0.24 +0.5 93,968 - BPHYT_RS00370 0.24 +1.0 94,009 + BPHYT_RS00370 0.29 +0.6 94,041 - BPHYT_RS00370 0.33 +0.8 94,442 + BPHYT_RS00370 0.83 -1.7 94,681 - BPHYT_RS00375 0.11 -0.1 94,874 + BPHYT_RS00375 0.32 +0.7 95,133 + BPHYT_RS00375 0.60 -1.6 95,221 - BPHYT_RS00375 0.70 +2.4 95,397 + BPHYT_RS00375 0.89 +0.2 95,398 - BPHYT_RS00375 0.89 -1.9 95,405 + BPHYT_RS00375 0.90 +1.4 95,406 - BPHYT_RS00375 0.90 -0.6 95,504 - +0.8 95,704 - BPHYT_RS00380 0.12 +0.2 95,965 - BPHYT_RS00380 0.56 -0.5 95,965 - BPHYT_RS00380 0.56 +0.2 96,164 - BPHYT_RS00380 0.89 -0.8 96,382 - BPHYT_RS00385 0.12 -1.0 96,382 - BPHYT_RS00385 0.12 -0.5 96,419 - BPHYT_RS00385 0.15 -0.4 96,468 + BPHYT_RS00385 0.18 -1.2 96,468 + BPHYT_RS00385 0.18 -0.3 96,468 + BPHYT_RS00385 0.18 +0.3 96,469 - BPHYT_RS00385 0.18 -1.0 96,469 - BPHYT_RS00385 0.18 +0.3
Or see this region's nucleotide sequence