Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35990 and BPHYT_RS35995 are separated by 197 nucleotides BPHYT_RS35995 and BPHYT_RS36000 are separated by 242 nucleotides BPHYT_RS36000 and BPHYT_RS36005 are separated by 151 nucleotides BPHYT_RS36005 and BPHYT_RS36010 are separated by 63 nucleotides
BPHYT_RS35990: BPHYT_RS35990 - hypothetical protein, at 31,778 to 31,987
_RS35990
BPHYT_RS35995: BPHYT_RS35995 - hypothetical protein, at 32,185 to 32,418
_RS35995
BPHYT_RS36000: BPHYT_RS36000 - hypothetical protein, at 32,661 to 32,903
_RS36000
BPHYT_RS36005: BPHYT_RS36005 - hypothetical protein, at 33,055 to 33,375
_RS36005
BPHYT_RS36010: BPHYT_RS36010 - hypothetical protein, at 33,439 to 33,915
_RS36010
Position (kb) 32
33 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 31.700 kb on + strand at 31.779 kb on + strand at 31.780 kb on - strand at 32.054 kb on - strand at 32.125 kb on + strand at 32.172 kb on - strand at 32.286 kb on + strand, within BPHYT_RS35995 at 32.321 kb on + strand, within BPHYT_RS35995 at 32.330 kb on + strand, within BPHYT_RS35995 at 32.330 kb on + strand, within BPHYT_RS35995 at 32.331 kb on - strand, within BPHYT_RS35995 at 32.331 kb on - strand, within BPHYT_RS35995 at 32.380 kb on + strand, within BPHYT_RS35995 at 32.487 kb on + strand at 32.488 kb on - strand at 32.501 kb on - strand at 32.814 kb on - strand, within BPHYT_RS36000 at 32.829 kb on - strand, within BPHYT_RS36000 at 32.829 kb on - strand, within BPHYT_RS36000 at 32.933 kb on - strand at 32.949 kb on + strand at 33.030 kb on + strand at 33.033 kb on + strand at 33.033 kb on + strand at 33.109 kb on + strand, within BPHYT_RS36005 at 33.110 kb on - strand, within BPHYT_RS36005 at 33.110 kb on - strand, within BPHYT_RS36005 at 33.239 kb on - strand at 33.239 kb on - strand, within BPHYT_RS36005 at 33.329 kb on - strand, within BPHYT_RS36005 at 33.358 kb on + strand at 33.359 kb on - strand at 33.359 kb on - strand at 33.560 kb on + strand, within BPHYT_RS36010 at 33.774 kb on + strand, within BPHYT_RS36010 at 33.775 kb on - strand, within BPHYT_RS36010 at 33.787 kb on + strand, within BPHYT_RS36010 at 33.787 kb on + strand, within BPHYT_RS36010 at 33.787 kb on + strand, within BPHYT_RS36010 at 33.788 kb on - strand, within BPHYT_RS36010 at 33.847 kb on + strand, within BPHYT_RS36010 at 33.902 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 31,700 + +0.2 31,779 + +0.4 31,780 - +0.0 32,054 - -0.5 32,125 + +1.0 32,172 - +0.2 32,286 + BPHYT_RS35995 0.43 -0.6 32,321 + BPHYT_RS35995 0.58 +0.0 32,330 + BPHYT_RS35995 0.62 -1.0 32,330 + BPHYT_RS35995 0.62 +0.3 32,331 - BPHYT_RS35995 0.62 -0.2 32,331 - BPHYT_RS35995 0.62 -1.3 32,380 + BPHYT_RS35995 0.83 +0.2 32,487 + +2.7 32,488 - +0.3 32,501 - -0.3 32,814 - BPHYT_RS36000 0.63 -2.0 32,829 - BPHYT_RS36000 0.69 -0.5 32,829 - BPHYT_RS36000 0.69 -1.4 32,933 - +1.3 32,949 + -2.7 33,030 + -0.2 33,033 + +0.4 33,033 + +0.6 33,109 + BPHYT_RS36005 0.17 +0.8 33,110 - BPHYT_RS36005 0.17 +0.2 33,110 - BPHYT_RS36005 0.17 -1.8 33,239 - +2.6 33,239 - BPHYT_RS36005 0.57 +1.0 33,329 - BPHYT_RS36005 0.85 -0.1 33,358 + +3.2 33,359 - -0.1 33,359 - -0.1 33,560 + BPHYT_RS36010 0.25 -0.2 33,774 + BPHYT_RS36010 0.70 -0.3 33,775 - BPHYT_RS36010 0.70 +1.3 33,787 + BPHYT_RS36010 0.73 -0.7 33,787 + BPHYT_RS36010 0.73 -0.4 33,787 + BPHYT_RS36010 0.73 +1.7 33,788 - BPHYT_RS36010 0.73 -2.1 33,847 + BPHYT_RS36010 0.86 -1.1 33,902 + -0.1
Or see this region's nucleotide sequence