Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35470 and BPHYT_RS35475 overlap by 4 nucleotides BPHYT_RS35475 and BPHYT_RS35480 overlap by 4 nucleotides BPHYT_RS35480 and BPHYT_RS35485 are separated by 293 nucleotides
BPHYT_RS35470: BPHYT_RS35470 - MarR family transcriptional regulator, at 3,558,214 to 3,558,777
_RS35470
BPHYT_RS35475: BPHYT_RS35475 - 3-hydroxyacyl-CoA dehydrogenase, at 3,558,774 to 3,559,547
_RS35475
BPHYT_RS35480: BPHYT_RS35480 - salicylyl-CoA 5-hydroxylase, at 3,559,544 to 3,561,916
_RS35480
BPHYT_RS35485: BPHYT_RS35485 - hypothetical protein, at 3,562,210 to 3,563,556
_RS35485
Position (kb) 3559
3560
3561
3562 Strain fitness (log2 ratio) -1
0
1
2
3 at 3560.304 kb on + strand, within BPHYT_RS35480 at 3560.305 kb on - strand, within BPHYT_RS35480 at 3560.869 kb on + strand, within BPHYT_RS35480 at 3562.111 kb on - strand at 3562.126 kb on - strand at 3562.130 kb on - strand at 3562.256 kb on + strand at 3562.345 kb on + strand, within BPHYT_RS35485 at 3562.345 kb on + strand, within BPHYT_RS35485
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 3,560,304 + BPHYT_RS35480 0.32 -0.6 3,560,305 - BPHYT_RS35480 0.32 +1.8 3,560,869 + BPHYT_RS35480 0.56 +3.2 3,562,111 - -0.4 3,562,126 - -1.0 3,562,130 - +1.9 3,562,256 + +0.0 3,562,345 + BPHYT_RS35485 0.10 -1.0 3,562,345 + BPHYT_RS35485 0.10 +0.3
Or see this region's nucleotide sequence