Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35295 and BPHYT_RS35300 are separated by 186 nucleotides BPHYT_RS35300 and BPHYT_RS35305 are separated by 24 nucleotides BPHYT_RS35305 and BPHYT_RS35310 are separated by 52 nucleotides
BPHYT_RS35295: BPHYT_RS35295 - 3'-kinase, at 3,518,889 to 3,519,692
_RS35295
BPHYT_RS35300: BPHYT_RS35300 - flavin reductase, at 3,519,879 to 3,520,463
_RS35300
BPHYT_RS35305: BPHYT_RS35305 - low molecular weight phosphotyrosine protein phosphatase, at 3,520,488 to 3,520,817
_RS35305
BPHYT_RS35310: BPHYT_RS35310 - GCN5 family N-acetyltransferase, at 3,520,870 to 3,521,355
_RS35310
Position (kb)
3519
3520
3521 Strain fitness (log2 ratio)
-2
-1
0
1 at 3518.882 kb on + strand at 3519.136 kb on + strand, within BPHYT_RS35295 at 3519.680 kb on - strand at 3519.715 kb on + strand at 3519.716 kb on - strand at 3519.875 kb on - strand at 3519.875 kb on - strand at 3520.314 kb on + strand, within BPHYT_RS35300 at 3520.386 kb on + strand, within BPHYT_RS35300 at 3520.387 kb on - strand, within BPHYT_RS35300 at 3520.489 kb on + strand at 3520.490 kb on - strand at 3520.490 kb on - strand at 3520.490 kb on - strand at 3520.558 kb on + strand, within BPHYT_RS35305 at 3520.581 kb on + strand, within BPHYT_RS35305 at 3520.581 kb on + strand, within BPHYT_RS35305 at 3520.812 kb on + strand at 3520.961 kb on + strand, within BPHYT_RS35310 at 3520.962 kb on - strand, within BPHYT_RS35310 at 3521.111 kb on + strand, within BPHYT_RS35310 at 3521.429 kb on - strand at 3521.429 kb on - strand at 3521.436 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 3,518,882 + +0.7 3,519,136 + BPHYT_RS35295 0.31 -1.3 3,519,680 - -0.4 3,519,715 + +0.3 3,519,716 - +0.3 3,519,875 - -0.1 3,519,875 - -0.1 3,520,314 + BPHYT_RS35300 0.74 -1.3 3,520,386 + BPHYT_RS35300 0.87 -1.1 3,520,387 - BPHYT_RS35300 0.87 -0.9 3,520,489 + -0.2 3,520,490 - -1.4 3,520,490 - -0.3 3,520,490 - +0.5 3,520,558 + BPHYT_RS35305 0.21 -1.8 3,520,581 + BPHYT_RS35305 0.28 -0.4 3,520,581 + BPHYT_RS35305 0.28 +0.3 3,520,812 + -1.3 3,520,961 + BPHYT_RS35310 0.19 +1.4 3,520,962 - BPHYT_RS35310 0.19 -0.5 3,521,111 + BPHYT_RS35310 0.50 -0.1 3,521,429 - +1.1 3,521,429 - +0.4 3,521,436 - -1.5
Or see this region's nucleotide sequence