Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS33585

Experiment: 36hr PHYTO4 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS33575 and BPHYT_RS33580 are separated by 85 nucleotidesBPHYT_RS33580 and BPHYT_RS33585 overlap by 4 nucleotidesBPHYT_RS33585 and BPHYT_RS33590 are separated by 286 nucleotides BPHYT_RS33575: BPHYT_RS33575 - 1-deoxy-D-xylulose-5-phosphate synthase, at 3,116,821 to 3,118,728 _RS33575 BPHYT_RS33580: BPHYT_RS33580 - geranyltranstransferase, at 3,118,814 to 3,119,695 _RS33580 BPHYT_RS33585: BPHYT_RS33585 - exodeoxyribonuclease VII small subunit, at 3,119,692 to 3,120,000 _RS33585 BPHYT_RS33590: BPHYT_RS33590 - Rieske (2Fe-2S) protein, at 3,120,287 to 3,121,393 _RS33590 Position (kb) 3119 3120 3121Strain fitness (log2 ratio) -2 -1 0 1 2at 3118.743 kb on - strandat 3118.770 kb on - strandat 3118.770 kb on - strandat 3118.770 kb on - strandat 3118.770 kb on - strandat 3118.770 kb on - strandat 3118.770 kb on - strandat 3118.783 kb on - strandat 3118.957 kb on - strand, within BPHYT_RS33580at 3118.957 kb on - strand, within BPHYT_RS33580at 3119.074 kb on - strand, within BPHYT_RS33580at 3119.383 kb on - strand, within BPHYT_RS33580at 3119.394 kb on - strandat 3119.394 kb on - strand, within BPHYT_RS33580at 3119.461 kb on - strand, within BPHYT_RS33580at 3119.461 kb on - strand, within BPHYT_RS33580at 3119.461 kb on - strand, within BPHYT_RS33580at 3119.635 kb on - strandat 3119.696 kb on - strandat 3119.696 kb on - strandat 3119.696 kb on - strandat 3119.696 kb on - strandat 3119.734 kb on - strand, within BPHYT_RS33585at 3120.005 kb on - strandat 3120.017 kb on - strandat 3120.017 kb on - strandat 3120.183 kb on + strandat 3120.210 kb on + strandat 3120.210 kb on + strandat 3120.228 kb on - strandat 3120.351 kb on - strandat 3120.351 kb on - strandat 3120.411 kb on - strand, within BPHYT_RS33590at 3120.608 kb on + strand, within BPHYT_RS33590at 3120.608 kb on + strand, within BPHYT_RS33590at 3120.609 kb on - strand, within BPHYT_RS33590at 3120.818 kb on + strand, within BPHYT_RS33590at 3120.818 kb on + strand, within BPHYT_RS33590at 3120.818 kb on + strand, within BPHYT_RS33590at 3120.819 kb on - strand, within BPHYT_RS33590at 3120.819 kb on - strand, within BPHYT_RS33590at 3120.857 kb on + strand, within BPHYT_RS33590at 3120.857 kb on + strand, within BPHYT_RS33590at 3120.858 kb on - strand, within BPHYT_RS33590at 3120.935 kb on + strand, within BPHYT_RS33590at 3120.936 kb on - strand, within BPHYT_RS33590at 3120.993 kb on - strand, within BPHYT_RS33590

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1
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3,118,743 - -0.7
3,118,770 - +0.5
3,118,770 - +0.5
3,118,770 - +0.0
3,118,770 - +0.4
3,118,770 - -0.3
3,118,770 - -0.1
3,118,783 - -0.6
3,118,957 - BPHYT_RS33580 0.16 -0.4
3,118,957 - BPHYT_RS33580 0.16 +2.2
3,119,074 - BPHYT_RS33580 0.29 -0.9
3,119,383 - BPHYT_RS33580 0.65 -0.1
3,119,394 - +0.5
3,119,394 - BPHYT_RS33580 0.66 -0.6
3,119,461 - BPHYT_RS33580 0.73 -2.7
3,119,461 - BPHYT_RS33580 0.73 +1.2
3,119,461 - BPHYT_RS33580 0.73 +0.1
3,119,635 - -1.7
3,119,696 - -1.7
3,119,696 - +1.8
3,119,696 - +1.5
3,119,696 - +1.0
3,119,734 - BPHYT_RS33585 0.14 -1.2
3,120,005 - +0.6
3,120,017 - -2.0
3,120,017 - +0.6
3,120,183 + +0.0
3,120,210 + -1.2
3,120,210 + -2.2
3,120,228 - +0.1
3,120,351 - -0.5
3,120,351 - -2.0
3,120,411 - BPHYT_RS33590 0.11 +1.2
3,120,608 + BPHYT_RS33590 0.29 -0.5
3,120,608 + BPHYT_RS33590 0.29 -1.0
3,120,609 - BPHYT_RS33590 0.29 +0.2
3,120,818 + BPHYT_RS33590 0.48 -1.2
3,120,818 + BPHYT_RS33590 0.48 -0.3
3,120,818 + BPHYT_RS33590 0.48 -0.5
3,120,819 - BPHYT_RS33590 0.48 +0.2
3,120,819 - BPHYT_RS33590 0.48 -0.1
3,120,857 + BPHYT_RS33590 0.51 +0.2
3,120,857 + BPHYT_RS33590 0.51 -1.7
3,120,858 - BPHYT_RS33590 0.52 +0.1
3,120,935 + BPHYT_RS33590 0.59 -1.0
3,120,936 - BPHYT_RS33590 0.59 -0.6
3,120,993 - BPHYT_RS33590 0.64 -0.1

Or see this region's nucleotide sequence