Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS33380 and BPHYT_RS33385 are separated by 50 nucleotides BPHYT_RS33385 and BPHYT_RS33390 are separated by 18 nucleotides
BPHYT_RS33380: BPHYT_RS33380 - 2-nitropropane dioxygenase, at 3,072,246 to 3,073,295
_RS33380
BPHYT_RS33385: BPHYT_RS33385 - major facilitator superfamily protein, at 3,073,346 to 3,074,617
_RS33385
BPHYT_RS33390: BPHYT_RS33390 - phosphate ABC transporter substrate-binding protein, at 3,074,636 to 3,075,670
_RS33390
Position (kb)
3073
3074
3075 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 3072.576 kb on + strand, within BPHYT_RS33380 at 3073.102 kb on - strand, within BPHYT_RS33380 at 3073.111 kb on + strand, within BPHYT_RS33380 at 3073.111 kb on + strand, within BPHYT_RS33380 at 3073.144 kb on - strand, within BPHYT_RS33380 at 3073.155 kb on + strand, within BPHYT_RS33380 at 3073.403 kb on + strand at 3073.403 kb on + strand at 3073.511 kb on + strand, within BPHYT_RS33385 at 3074.451 kb on - strand, within BPHYT_RS33385 at 3074.728 kb on + strand at 3074.838 kb on + strand, within BPHYT_RS33390 at 3074.839 kb on - strand, within BPHYT_RS33390 at 3074.998 kb on + strand, within BPHYT_RS33390 at 3075.056 kb on + strand, within BPHYT_RS33390 at 3075.056 kb on + strand, within BPHYT_RS33390 at 3075.093 kb on - strand, within BPHYT_RS33390 at 3075.297 kb on + strand, within BPHYT_RS33390 at 3075.298 kb on - strand, within BPHYT_RS33390 at 3075.298 kb on - strand, within BPHYT_RS33390 at 3075.346 kb on + strand, within BPHYT_RS33390 at 3075.599 kb on - strand at 3075.600 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 3,072,576 + BPHYT_RS33380 0.31 +0.8 3,073,102 - BPHYT_RS33380 0.82 -0.7 3,073,111 + BPHYT_RS33380 0.82 -2.2 3,073,111 + BPHYT_RS33380 0.82 +1.8 3,073,144 - BPHYT_RS33380 0.86 -0.9 3,073,155 + BPHYT_RS33380 0.87 -0.5 3,073,403 + +0.3 3,073,403 + -0.6 3,073,511 + BPHYT_RS33385 0.13 -1.3 3,074,451 - BPHYT_RS33385 0.87 -1.4 3,074,728 + -2.5 3,074,838 + BPHYT_RS33390 0.20 +0.2 3,074,839 - BPHYT_RS33390 0.20 +0.2 3,074,998 + BPHYT_RS33390 0.35 +1.5 3,075,056 + BPHYT_RS33390 0.41 -1.2 3,075,056 + BPHYT_RS33390 0.41 -0.5 3,075,093 - BPHYT_RS33390 0.44 -1.3 3,075,297 + BPHYT_RS33390 0.64 -0.4 3,075,298 - BPHYT_RS33390 0.64 +5.5 3,075,298 - BPHYT_RS33390 0.64 -0.4 3,075,346 + BPHYT_RS33390 0.69 -0.9 3,075,599 - +0.3 3,075,600 + -0.2
Or see this region's nucleotide sequence