Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS33155

Experiment: 36hr PHYTO4 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS33150 and BPHYT_RS33155 are separated by 467 nucleotidesBPHYT_RS33155 and BPHYT_RS33160 are separated by 381 nucleotides BPHYT_RS33150: BPHYT_RS33150 - beta-lactamase, at 3,014,085 to 3,015,017 _RS33150 BPHYT_RS33155: BPHYT_RS33155 - hypothetical protein, at 3,015,485 to 3,015,670 _RS33155 BPHYT_RS33160: BPHYT_RS33160 - hypothetical protein, at 3,016,052 to 3,016,570 _RS33160 Position (kb) 3015 3016Strain fitness (log2 ratio) -1 0 1 2at 3014.855 kb on + strand, within BPHYT_RS33150at 3015.038 kb on - strandat 3015.142 kb on + strandat 3015.252 kb on - strandat 3015.426 kb on + strandat 3015.427 kb on - strandat 3015.530 kb on - strand, within BPHYT_RS33155at 3015.575 kb on - strand, within BPHYT_RS33155at 3015.636 kb on + strand, within BPHYT_RS33155at 3015.773 kb on + strandat 3016.022 kb on + strandat 3016.096 kb on + strandat 3016.203 kb on - strand, within BPHYT_RS33160at 3016.319 kb on - strand, within BPHYT_RS33160at 3016.336 kb on - strand, within BPHYT_RS33160at 3016.466 kb on - strand, within BPHYT_RS33160at 3016.466 kb on - strand, within BPHYT_RS33160at 3016.573 kb on + strandat 3016.573 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1
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3,014,855 + BPHYT_RS33150 0.83 +1.7
3,015,038 - -0.7
3,015,142 + +0.2
3,015,252 - +0.2
3,015,426 + -0.6
3,015,427 - +0.2
3,015,530 - BPHYT_RS33155 0.24 -1.6
3,015,575 - BPHYT_RS33155 0.48 -0.6
3,015,636 + BPHYT_RS33155 0.81 -0.5
3,015,773 + +0.6
3,016,022 + +0.2
3,016,096 + +0.2
3,016,203 - BPHYT_RS33160 0.29 -1.0
3,016,319 - BPHYT_RS33160 0.51 -1.0
3,016,336 - BPHYT_RS33160 0.55 -0.3
3,016,466 - BPHYT_RS33160 0.80 +2.5
3,016,466 - BPHYT_RS33160 0.80 +0.5
3,016,573 + +1.5
3,016,573 + +0.1

Or see this region's nucleotide sequence