Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS33025 and BPHYT_RS33030 are separated by 135 nucleotides BPHYT_RS33030 and BPHYT_RS33035 are separated by 70 nucleotides BPHYT_RS33035 and BPHYT_RS33040 are separated by 156 nucleotides BPHYT_RS33040 and BPHYT_RS33045 are separated by 395 nucleotides
BPHYT_RS33025: BPHYT_RS33025 - hypothetical protein, at 2,987,263 to 2,988,075
_RS33025
BPHYT_RS33030: BPHYT_RS33030 - hypothetical protein, at 2,988,211 to 2,988,975
_RS33030
BPHYT_RS33035: BPHYT_RS33035 - murein transglycosylase, at 2,989,046 to 2,990,065
_RS33035
BPHYT_RS33040: BPHYT_RS33040 - hypothetical protein, at 2,990,222 to 2,990,440
_RS33040
BPHYT_RS33045: BPHYT_RS33045 - response regulator receiver protein, at 2,990,836 to 2,991,213
_RS33045
Position (kb)
2989
2990
2991 Strain fitness (log2 ratio)
-2
-1
0
1 at 2988.614 kb on + strand, within BPHYT_RS33030 at 2989.325 kb on + strand, within BPHYT_RS33035 at 2989.561 kb on + strand, within BPHYT_RS33035 at 2989.760 kb on + strand, within BPHYT_RS33035 at 2990.009 kb on + strand at 2990.010 kb on - strand at 2990.010 kb on - strand at 2990.199 kb on + strand at 2990.330 kb on + strand, within BPHYT_RS33040 at 2990.337 kb on - strand, within BPHYT_RS33040 at 2990.397 kb on - strand, within BPHYT_RS33040 at 2990.461 kb on + strand at 2990.484 kb on - strand at 2990.570 kb on - strand at 2990.626 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 2,988,614 + BPHYT_RS33030 0.53 -0.9 2,989,325 + BPHYT_RS33035 0.27 -1.9 2,989,561 + BPHYT_RS33035 0.50 -0.1 2,989,760 + BPHYT_RS33035 0.70 +0.2 2,990,009 + +0.2 2,990,010 - +0.6 2,990,010 - -1.8 2,990,199 + -0.2 2,990,330 + BPHYT_RS33040 0.49 +0.1 2,990,337 - BPHYT_RS33040 0.53 +1.0 2,990,397 - BPHYT_RS33040 0.80 -0.1 2,990,461 + -0.3 2,990,484 - -0.1 2,990,570 - +0.7 2,990,626 + +0.1
Or see this region's nucleotide sequence