Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS33005

Experiment: 36hr PHYTO4 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS32995 and BPHYT_RS33000 are separated by 69 nucleotidesBPHYT_RS33000 and BPHYT_RS33005 are separated by 95 nucleotidesBPHYT_RS33005 and BPHYT_RS33010 are separated by 72 nucleotidesBPHYT_RS33010 and BPHYT_RS33015 are separated by 117 nucleotides BPHYT_RS32995: BPHYT_RS32995 - hypothetical protein, at 2,981,897 to 2,983,363 _RS32995 BPHYT_RS33000: BPHYT_RS33000 - histidine biosynthesis protein, at 2,983,433 to 2,983,702 _RS33000 BPHYT_RS33005: BPHYT_RS33005 - AraC family transcriptional regulator, at 2,983,798 to 2,984,772 _RS33005 BPHYT_RS33010: BPHYT_RS33010 - hypothetical protein, at 2,984,845 to 2,985,345 _RS33010 BPHYT_RS33015: BPHYT_RS33015 - hypothetical protein, at 2,985,463 to 2,985,960 _RS33015 Position (kb) 2983 2984 2985Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2982.905 kb on - strand, within BPHYT_RS32995at 2982.915 kb on + strand, within BPHYT_RS32995at 2982.924 kb on + strand, within BPHYT_RS32995at 2983.003 kb on - strand, within BPHYT_RS32995at 2983.054 kb on - strand, within BPHYT_RS32995at 2983.054 kb on - strand, within BPHYT_RS32995at 2983.075 kb on - strand, within BPHYT_RS32995at 2983.735 kb on + strandat 2983.831 kb on + strandat 2984.058 kb on + strand, within BPHYT_RS33005at 2984.922 kb on - strand, within BPHYT_RS33010at 2984.978 kb on - strand, within BPHYT_RS33010at 2985.353 kb on - strandat 2985.422 kb on + strandat 2985.697 kb on + strand, within BPHYT_RS33015

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1
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2,982,905 - BPHYT_RS32995 0.69 +1.1
2,982,915 + BPHYT_RS32995 0.69 -1.1
2,982,924 + BPHYT_RS32995 0.70 -0.1
2,983,003 - BPHYT_RS32995 0.75 -2.8
2,983,054 - BPHYT_RS32995 0.79 +2.2
2,983,054 - BPHYT_RS32995 0.79 -0.6
2,983,075 - BPHYT_RS32995 0.80 +2.8
2,983,735 + -0.5
2,983,831 + +0.4
2,984,058 + BPHYT_RS33005 0.27 +0.5
2,984,922 - BPHYT_RS33010 0.15 +0.7
2,984,978 - BPHYT_RS33010 0.27 +0.6
2,985,353 - +0.5
2,985,422 + -0.3
2,985,697 + BPHYT_RS33015 0.47 +0.4

Or see this region's nucleotide sequence