Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS32995 and BPHYT_RS33000 are separated by 69 nucleotides BPHYT_RS33000 and BPHYT_RS33005 are separated by 95 nucleotides BPHYT_RS33005 and BPHYT_RS33010 are separated by 72 nucleotides BPHYT_RS33010 and BPHYT_RS33015 are separated by 117 nucleotides
BPHYT_RS32995: BPHYT_RS32995 - hypothetical protein, at 2,981,897 to 2,983,363
_RS32995
BPHYT_RS33000: BPHYT_RS33000 - histidine biosynthesis protein, at 2,983,433 to 2,983,702
_RS33000
BPHYT_RS33005: BPHYT_RS33005 - AraC family transcriptional regulator, at 2,983,798 to 2,984,772
_RS33005
BPHYT_RS33010: BPHYT_RS33010 - hypothetical protein, at 2,984,845 to 2,985,345
_RS33010
BPHYT_RS33015: BPHYT_RS33015 - hypothetical protein, at 2,985,463 to 2,985,960
_RS33015
Position (kb)
2983
2984
2985 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2982.905 kb on - strand, within BPHYT_RS32995 at 2982.915 kb on + strand, within BPHYT_RS32995 at 2982.924 kb on + strand, within BPHYT_RS32995 at 2983.003 kb on - strand, within BPHYT_RS32995 at 2983.054 kb on - strand, within BPHYT_RS32995 at 2983.054 kb on - strand, within BPHYT_RS32995 at 2983.075 kb on - strand, within BPHYT_RS32995 at 2983.735 kb on + strand at 2983.831 kb on + strand at 2984.058 kb on + strand, within BPHYT_RS33005 at 2984.922 kb on - strand, within BPHYT_RS33010 at 2984.978 kb on - strand, within BPHYT_RS33010 at 2985.353 kb on - strand at 2985.422 kb on + strand at 2985.697 kb on + strand, within BPHYT_RS33015
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 2,982,905 - BPHYT_RS32995 0.69 +1.1 2,982,915 + BPHYT_RS32995 0.69 -1.1 2,982,924 + BPHYT_RS32995 0.70 -0.1 2,983,003 - BPHYT_RS32995 0.75 -2.8 2,983,054 - BPHYT_RS32995 0.79 +2.2 2,983,054 - BPHYT_RS32995 0.79 -0.6 2,983,075 - BPHYT_RS32995 0.80 +2.8 2,983,735 + -0.5 2,983,831 + +0.4 2,984,058 + BPHYT_RS33005 0.27 +0.5 2,984,922 - BPHYT_RS33010 0.15 +0.7 2,984,978 - BPHYT_RS33010 0.27 +0.6 2,985,353 - +0.5 2,985,422 + -0.3 2,985,697 + BPHYT_RS33015 0.47 +0.4
Or see this region's nucleotide sequence