Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS32320

Experiment: 36hr PHYTO4 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS32315 and BPHYT_RS32320 overlap by 4 nucleotidesBPHYT_RS32320 and BPHYT_RS32325 are separated by 993 nucleotides BPHYT_RS32315: BPHYT_RS32315 - FAD-dependent oxidoreductase, at 2,819,758 to 2,820,882 _RS32315 BPHYT_RS32320: BPHYT_RS32320 - nopaline dehydrogenase, at 2,820,879 to 2,822,294 _RS32320 BPHYT_RS32325: BPHYT_RS32325 - 5-oxoprolinase, at 2,823,288 to 2,825,012 _RS32325 Position (kb) 2820 2821 2822 2823Strain fitness (log2 ratio) -1 0 1at 2820.155 kb on + strand, within BPHYT_RS32315at 2820.912 kb on + strandat 2820.989 kb on + strandat 2821.153 kb on - strand, within BPHYT_RS32320at 2821.488 kb on + strand, within BPHYT_RS32320at 2821.489 kb on - strand, within BPHYT_RS32320at 2821.802 kb on - strand, within BPHYT_RS32320at 2821.802 kb on - strand, within BPHYT_RS32320at 2822.004 kb on - strand, within BPHYT_RS32320at 2822.056 kb on + strand, within BPHYT_RS32320at 2822.832 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1
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2,820,155 + BPHYT_RS32315 0.35 +0.3
2,820,912 + +0.9
2,820,989 + -0.2
2,821,153 - BPHYT_RS32320 0.19 -0.9
2,821,488 + BPHYT_RS32320 0.43 -0.4
2,821,489 - BPHYT_RS32320 0.43 +0.6
2,821,802 - BPHYT_RS32320 0.65 -0.7
2,821,802 - BPHYT_RS32320 0.65 -1.1
2,822,004 - BPHYT_RS32320 0.79 -0.5
2,822,056 + BPHYT_RS32320 0.83 +0.5
2,822,832 - +0.1

Or see this region's nucleotide sequence