Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS31895 and BPHYT_RS31900 overlap by 26 nucleotides BPHYT_RS31900 and BPHYT_RS31905 are separated by 83 nucleotides BPHYT_RS31905 and BPHYT_RS31910 are separated by 489 nucleotides
BPHYT_RS31895: BPHYT_RS31895 - hypothetical protein, at 2,715,842 to 2,717,506
_RS31895
BPHYT_RS31900: BPHYT_RS31900 - short-chain dehydrogenase/reductase SDR, at 2,717,481 to 2,718,260
_RS31900
BPHYT_RS31905: BPHYT_RS31905 - hypothetical protein, at 2,718,344 to 2,718,865
_RS31905
BPHYT_RS31910: BPHYT_RS31910 - hypothetical protein, at 2,719,355 to 2,720,071
_RS31910
Position (kb)
2718
2719 Strain fitness (log2 ratio)
-1
0
1
2 at 2717.526 kb on - strand at 2717.712 kb on - strand, within BPHYT_RS31900 at 2718.059 kb on + strand, within BPHYT_RS31900 at 2718.328 kb on + strand at 2718.372 kb on + strand at 2718.451 kb on - strand, within BPHYT_RS31905 at 2718.839 kb on - strand at 2718.894 kb on + strand at 2719.103 kb on - strand at 2719.351 kb on + strand at 2719.547 kb on - strand, within BPHYT_RS31910 at 2719.620 kb on - strand, within BPHYT_RS31910
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 2,717,526 - +0.1 2,717,712 - BPHYT_RS31900 0.30 -1.5 2,718,059 + BPHYT_RS31900 0.74 -0.5 2,718,328 + +0.5 2,718,372 + -0.4 2,718,451 - BPHYT_RS31905 0.20 -1.6 2,718,839 - +0.5 2,718,894 + -1.4 2,719,103 - -0.6 2,719,351 + +1.9 2,719,547 - BPHYT_RS31910 0.27 -1.1 2,719,620 - BPHYT_RS31910 0.37 +0.3
Or see this region's nucleotide sequence