Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS31575 and BPHYT_RS31580 are separated by 365 nucleotides BPHYT_RS31580 and BPHYT_RS31585 are separated by 3 nucleotides BPHYT_RS31585 and BPHYT_RS31590 are separated by 60 nucleotides BPHYT_RS31590 and BPHYT_RS31595 are separated by 479 nucleotides
BPHYT_RS31575: BPHYT_RS31575 - hypothetical protein, at 2,637,896 to 2,638,225
_RS31575
BPHYT_RS31580: BPHYT_RS31580 - hypothetical protein, at 2,638,591 to 2,638,818
_RS31580
BPHYT_RS31585: BPHYT_RS31585 - universal stress protein UspA, at 2,638,822 to 2,639,256
_RS31585
BPHYT_RS31590: BPHYT_RS31590 - hypothetical protein, at 2,639,317 to 2,639,502
_RS31590
BPHYT_RS31595: BPHYT_RS31595 - hypothetical protein, at 2,639,982 to 2,640,674
_RS31595
Position (kb)
2638
2639
2640 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2637.866 kb on - strand at 2637.866 kb on - strand at 2637.870 kb on - strand at 2637.880 kb on - strand at 2637.905 kb on - strand at 2637.905 kb on - strand at 2637.933 kb on + strand, within BPHYT_RS31575 at 2637.974 kb on - strand, within BPHYT_RS31575 at 2637.994 kb on + strand, within BPHYT_RS31575 at 2637.997 kb on - strand, within BPHYT_RS31575 at 2638.208 kb on - strand at 2638.211 kb on - strand at 2638.224 kb on - strand at 2638.452 kb on + strand at 2638.453 kb on - strand at 2638.824 kb on - strand at 2638.856 kb on - strand at 2639.053 kb on + strand, within BPHYT_RS31585 at 2639.195 kb on - strand, within BPHYT_RS31585 at 2639.195 kb on - strand, within BPHYT_RS31585 at 2639.347 kb on + strand, within BPHYT_RS31590 at 2639.626 kb on - strand at 2639.654 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 2,637,866 - +0.1 2,637,866 - -0.2 2,637,870 - -0.2 2,637,880 - +1.0 2,637,905 - -3.7 2,637,905 - -1.0 2,637,933 + BPHYT_RS31575 0.11 -0.3 2,637,974 - BPHYT_RS31575 0.24 -0.8 2,637,994 + BPHYT_RS31575 0.30 -0.2 2,637,997 - BPHYT_RS31575 0.31 -0.5 2,638,208 - +0.8 2,638,211 - +1.5 2,638,224 - +0.8 2,638,452 + -0.9 2,638,453 - +1.6 2,638,824 - +0.9 2,638,856 - +0.5 2,639,053 + BPHYT_RS31585 0.53 -1.1 2,639,195 - BPHYT_RS31585 0.86 -0.1 2,639,195 - BPHYT_RS31585 0.86 -1.5 2,639,347 + BPHYT_RS31590 0.16 +0.7 2,639,626 - -0.6 2,639,654 + +0.1
Or see this region's nucleotide sequence