Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS31540 and BPHYT_RS31545 are separated by 125 nucleotides BPHYT_RS31545 and BPHYT_RS31550 are separated by 441 nucleotides
BPHYT_RS31540: BPHYT_RS31540 - chloride channel protein, at 2,629,423 to 2,630,748
_RS31540
BPHYT_RS31545: BPHYT_RS31545 - membrane protein, at 2,630,874 to 2,631,539
_RS31545
BPHYT_RS31550: BPHYT_RS31550 - hypothetical protein, at 2,631,981 to 2,632,217
_RS31550
Position (kb)
2630
2631
2632 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 2630.085 kb on - strand, within BPHYT_RS31540 at 2630.546 kb on - strand, within BPHYT_RS31540 at 2630.548 kb on - strand, within BPHYT_RS31540 at 2630.685 kb on - strand at 2630.781 kb on - strand at 2631.318 kb on - strand, within BPHYT_RS31545 at 2631.423 kb on + strand, within BPHYT_RS31545 at 2631.424 kb on - strand, within BPHYT_RS31545 at 2631.424 kb on - strand, within BPHYT_RS31545 at 2631.424 kb on - strand, within BPHYT_RS31545 at 2631.456 kb on + strand, within BPHYT_RS31545 at 2631.539 kb on + strand at 2631.542 kb on - strand at 2631.542 kb on - strand at 2631.542 kb on - strand at 2631.653 kb on + strand at 2631.654 kb on - strand at 2631.833 kb on - strand at 2632.122 kb on + strand, within BPHYT_RS31550 at 2632.123 kb on - strand, within BPHYT_RS31550 at 2632.137 kb on - strand, within BPHYT_RS31550 at 2632.155 kb on + strand, within BPHYT_RS31550 at 2632.223 kb on - strand at 2632.449 kb on + strand at 2632.449 kb on + strand at 2632.450 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 2,630,085 - BPHYT_RS31540 0.50 -1.5 2,630,546 - BPHYT_RS31540 0.85 -0.2 2,630,548 - BPHYT_RS31540 0.85 -1.8 2,630,685 - +0.7 2,630,781 - +0.2 2,631,318 - BPHYT_RS31545 0.67 -1.7 2,631,423 + BPHYT_RS31545 0.82 +0.0 2,631,424 - BPHYT_RS31545 0.83 +1.4 2,631,424 - BPHYT_RS31545 0.83 -0.5 2,631,424 - BPHYT_RS31545 0.83 -1.9 2,631,456 + BPHYT_RS31545 0.87 -1.0 2,631,539 + -0.5 2,631,542 - -0.5 2,631,542 - +2.6 2,631,542 - +1.0 2,631,653 + -1.0 2,631,654 - +1.6 2,631,833 - -0.9 2,632,122 + BPHYT_RS31550 0.59 +5.4 2,632,123 - BPHYT_RS31550 0.60 +1.8 2,632,137 - BPHYT_RS31550 0.66 +1.2 2,632,155 + BPHYT_RS31550 0.73 -0.7 2,632,223 - -0.5 2,632,449 + -0.7 2,632,449 + +1.4 2,632,450 - +0.4
Or see this region's nucleotide sequence