Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS31375 and BPHYT_RS31380 are separated by 102 nucleotides BPHYT_RS31380 and BPHYT_RS31385 are separated by 63 nucleotides BPHYT_RS31385 and BPHYT_RS31390 overlap by 7 nucleotides BPHYT_RS31390 and BPHYT_RS31395 are separated by 148 nucleotides
BPHYT_RS31375: BPHYT_RS31375 - AraC family transcriptional regulator, at 2,593,776 to 2,594,693
_RS31375
BPHYT_RS31380: BPHYT_RS31380 - dehydrogenase, at 2,594,796 to 2,595,545
_RS31380
BPHYT_RS31385: BPHYT_RS31385 - ketoacyl reductase, at 2,595,609 to 2,596,367
_RS31385
BPHYT_RS31390: BPHYT_RS31390 - hypothetical protein, at 2,596,361 to 2,596,591
_RS31390
BPHYT_RS31395: BPHYT_RS31395 - thiamine pyrophosphate-binding protein, at 2,596,740 to 2,598,488
_RS31395
Position (kb)
2595
2596
2597 Strain fitness (log2 ratio)
-2
-1
0
1 at 2594.806 kb on + strand at 2595.574 kb on + strand at 2595.591 kb on + strand at 2595.635 kb on - strand at 2596.128 kb on + strand, within BPHYT_RS31385 at 2596.249 kb on - strand, within BPHYT_RS31385 at 2596.483 kb on + strand, within BPHYT_RS31390 at 2596.484 kb on - strand, within BPHYT_RS31390 at 2596.549 kb on + strand, within BPHYT_RS31390 at 2596.800 kb on + strand at 2596.800 kb on + strand at 2597.032 kb on + strand, within BPHYT_RS31395 at 2597.183 kb on - strand, within BPHYT_RS31395
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 2,594,806 + -0.7 2,595,574 + +1.1 2,595,591 + +0.0 2,595,635 - -0.2 2,596,128 + BPHYT_RS31385 0.68 -1.1 2,596,249 - BPHYT_RS31385 0.84 -1.9 2,596,483 + BPHYT_RS31390 0.53 -0.6 2,596,484 - BPHYT_RS31390 0.53 -0.4 2,596,549 + BPHYT_RS31390 0.81 +0.3 2,596,800 + -0.3 2,596,800 + +0.6 2,597,032 + BPHYT_RS31395 0.17 -0.6 2,597,183 - BPHYT_RS31395 0.25 +0.6
Or see this region's nucleotide sequence