Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS30540 and BPHYT_RS30545 are separated by 85 nucleotides BPHYT_RS30545 and BPHYT_RS30550 overlap by 4 nucleotides BPHYT_RS30550 and BPHYT_RS30555 are separated by 208 nucleotides BPHYT_RS30555 and BPHYT_RS30560 are separated by 86 nucleotides
BPHYT_RS30540: BPHYT_RS30540 - hypothetical protein, at 2,412,040 to 2,412,285
_RS30540
BPHYT_RS30545: BPHYT_RS30545 - hypothetical protein, at 2,412,371 to 2,412,661
_RS30545
BPHYT_RS30550: BPHYT_RS30550 - hypothetical protein, at 2,412,658 to 2,412,858
_RS30550
BPHYT_RS30555: BPHYT_RS30555 - glutathione S-transferase, at 2,413,067 to 2,413,747
_RS30555
BPHYT_RS30560: BPHYT_RS30560 - transcriptional regulator, at 2,413,834 to 2,415,429
_RS30560
Position (kb)
2413
2414 Strain fitness (log2 ratio)
-1
0
1
2 at 2413.047 kb on - strand at 2413.160 kb on - strand, within BPHYT_RS30555 at 2413.499 kb on + strand, within BPHYT_RS30555 at 2413.499 kb on + strand, within BPHYT_RS30555 at 2413.517 kb on + strand, within BPHYT_RS30555 at 2413.566 kb on - strand, within BPHYT_RS30555 at 2413.595 kb on + strand, within BPHYT_RS30555 at 2413.717 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 2,413,047 - -0.4 2,413,160 - BPHYT_RS30555 0.14 +0.0 2,413,499 + BPHYT_RS30555 0.63 +2.0 2,413,499 + BPHYT_RS30555 0.63 -1.2 2,413,517 + BPHYT_RS30555 0.66 +0.3 2,413,566 - BPHYT_RS30555 0.73 +0.7 2,413,595 + BPHYT_RS30555 0.78 -1.6 2,413,717 - -0.8
Or see this region's nucleotide sequence