Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS29750 and BPHYT_RS29755 are separated by 603 nucleotides
BPHYT_RS29750: BPHYT_RS29750 - phosphoenolpyruvate phosphomutase, at 2,227,181 to 2,228,926
_RS29750
BPHYT_RS29755: BPHYT_RS29755 - membrane protein, at 2,229,530 to 2,231,071
_RS29755
Position (kb)
2229
2230
2231
2232 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2228.749 kb on - strand, within BPHYT_RS29750 at 2228.749 kb on - strand, within BPHYT_RS29750 at 2228.929 kb on + strand at 2228.929 kb on + strand at 2228.933 kb on + strand at 2228.934 kb on - strand at 2228.934 kb on - strand at 2228.965 kb on + strand at 2228.966 kb on - strand at 2229.451 kb on + strand at 2229.693 kb on - strand, within BPHYT_RS29755 at 2229.693 kb on - strand, within BPHYT_RS29755 at 2229.788 kb on + strand, within BPHYT_RS29755 at 2229.947 kb on + strand, within BPHYT_RS29755 at 2229.947 kb on + strand, within BPHYT_RS29755 at 2229.947 kb on + strand, within BPHYT_RS29755 at 2229.948 kb on - strand, within BPHYT_RS29755 at 2229.996 kb on - strand, within BPHYT_RS29755 at 2230.025 kb on + strand, within BPHYT_RS29755 at 2230.043 kb on + strand, within BPHYT_RS29755 at 2230.043 kb on + strand, within BPHYT_RS29755 at 2230.043 kb on + strand, within BPHYT_RS29755 at 2230.043 kb on + strand, within BPHYT_RS29755 at 2230.044 kb on - strand, within BPHYT_RS29755 at 2230.044 kb on - strand, within BPHYT_RS29755 at 2230.044 kb on - strand, within BPHYT_RS29755 at 2230.044 kb on - strand, within BPHYT_RS29755 at 2230.099 kb on + strand, within BPHYT_RS29755 at 2230.099 kb on + strand, within BPHYT_RS29755 at 2230.154 kb on - strand, within BPHYT_RS29755 at 2231.338 kb on - strand at 2231.338 kb on - strand at 2231.502 kb on + strand at 2231.680 kb on + strand at 2231.683 kb on + strand at 2231.684 kb on - strand at 2231.684 kb on - strand at 2231.960 kb on + strand at 2231.960 kb on + strand at 2231.962 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 2,228,749 - BPHYT_RS29750 0.90 +2.6 2,228,749 - BPHYT_RS29750 0.90 +0.4 2,228,929 + -0.4 2,228,929 + -0.3 2,228,933 + +1.2 2,228,934 - -0.1 2,228,934 - -0.2 2,228,965 + +0.8 2,228,966 - -0.1 2,229,451 + +0.5 2,229,693 - BPHYT_RS29755 0.11 +1.6 2,229,693 - BPHYT_RS29755 0.11 +0.3 2,229,788 + BPHYT_RS29755 0.17 -2.0 2,229,947 + BPHYT_RS29755 0.27 +1.8 2,229,947 + BPHYT_RS29755 0.27 +1.1 2,229,947 + BPHYT_RS29755 0.27 -1.0 2,229,948 - BPHYT_RS29755 0.27 +2.6 2,229,996 - BPHYT_RS29755 0.30 +2.9 2,230,025 + BPHYT_RS29755 0.32 -0.6 2,230,043 + BPHYT_RS29755 0.33 -2.3 2,230,043 + BPHYT_RS29755 0.33 +0.5 2,230,043 + BPHYT_RS29755 0.33 -1.2 2,230,043 + BPHYT_RS29755 0.33 +2.0 2,230,044 - BPHYT_RS29755 0.33 -0.7 2,230,044 - BPHYT_RS29755 0.33 -1.2 2,230,044 - BPHYT_RS29755 0.33 -1.3 2,230,044 - BPHYT_RS29755 0.33 -1.2 2,230,099 + BPHYT_RS29755 0.37 +1.4 2,230,099 + BPHYT_RS29755 0.37 -1.1 2,230,154 - BPHYT_RS29755 0.40 -0.1 2,231,338 - +1.5 2,231,338 - -0.4 2,231,502 + -1.0 2,231,680 + +0.6 2,231,683 + -0.2 2,231,684 - +1.6 2,231,684 - -0.1 2,231,960 + -0.6 2,231,960 + +0.6 2,231,962 + -1.0
Or see this region's nucleotide sequence