Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS29170 and BPHYT_RS29175 are separated by 50 nucleotides BPHYT_RS29175 and BPHYT_RS29180 are separated by 13 nucleotides BPHYT_RS29180 and BPHYT_RS29185 overlap by 1 nucleotides
BPHYT_RS29170: BPHYT_RS29170 - LacI family transcriptional regulator, at 2,100,100 to 2,101,143
_RS29170
BPHYT_RS29175: BPHYT_RS29175 - ABC transporter ATP-binding protein, at 2,101,194 to 2,102,366
_RS29175
BPHYT_RS29180: BPHYT_RS29180 - sugar ABC transporter permease, at 2,102,380 to 2,103,300
_RS29180
BPHYT_RS29185: BPHYT_RS29185 - sugar ABC transporter permease, at 2,103,300 to 2,104,244
_RS29185
Position (kb)
2101
2102
2103 Strain fitness (log2 ratio)
-1
0
1
2 at 2100.924 kb on - strand, within BPHYT_RS29170 at 2101.168 kb on - strand at 2101.168 kb on - strand at 2101.197 kb on + strand at 2101.239 kb on - strand at 2101.702 kb on + strand, within BPHYT_RS29175 at 2101.703 kb on - strand, within BPHYT_RS29175 at 2102.008 kb on + strand, within BPHYT_RS29175 at 2102.361 kb on - strand at 2102.439 kb on - strand at 2102.491 kb on - strand, within BPHYT_RS29180 at 2102.492 kb on + strand, within BPHYT_RS29180 at 2102.732 kb on + strand, within BPHYT_RS29180 at 2103.155 kb on + strand, within BPHYT_RS29180
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 2,100,924 - BPHYT_RS29170 0.79 +2.3 2,101,168 - +0.4 2,101,168 - -0.9 2,101,197 + +0.1 2,101,239 - -0.4 2,101,702 + BPHYT_RS29175 0.43 -0.7 2,101,703 - BPHYT_RS29175 0.43 -1.4 2,102,008 + BPHYT_RS29175 0.69 +1.1 2,102,361 - +0.9 2,102,439 - +0.5 2,102,491 - BPHYT_RS29180 0.12 -0.6 2,102,492 + BPHYT_RS29180 0.12 +0.4 2,102,732 + BPHYT_RS29180 0.38 -0.9 2,103,155 + BPHYT_RS29180 0.84 +1.0
Or see this region's nucleotide sequence