Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28525 and BPHYT_RS28530 are separated by 28 nucleotides BPHYT_RS28530 and BPHYT_RS28535 are separated by 58 nucleotides BPHYT_RS28535 and BPHYT_RS28540 are separated by 1 nucleotides
BPHYT_RS28525: BPHYT_RS28525 - amidase, at 1,955,720 to 1,957,240
_RS28525
BPHYT_RS28530: BPHYT_RS28530 - 3-ketoacyl-ACP reductase, at 1,957,269 to 1,958,033
_RS28530
BPHYT_RS28535: BPHYT_RS28535 - response regulator receiver, at 1,958,092 to 1,958,742
_RS28535
BPHYT_RS28540: BPHYT_RS28540 - amino acid ABC substrate-binding protein, at 1,958,744 to 1,959,898
_RS28540
Position (kb)
1958
1959 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1957.266 kb on - strand at 1957.837 kb on - strand, within BPHYT_RS28530 at 1958.100 kb on + strand at 1958.466 kb on - strand, within BPHYT_RS28535 at 1958.749 kb on - strand at 1958.800 kb on + strand at 1958.800 kb on + strand at 1958.803 kb on - strand at 1959.017 kb on - strand, within BPHYT_RS28540 at 1959.017 kb on - strand at 1959.250 kb on - strand, within BPHYT_RS28540 at 1959.264 kb on + strand, within BPHYT_RS28540 at 1959.373 kb on - strand, within BPHYT_RS28540 at 1959.453 kb on + strand, within BPHYT_RS28540 at 1959.472 kb on - strand, within BPHYT_RS28540 at 1959.546 kb on + strand, within BPHYT_RS28540 at 1959.652 kb on + strand, within BPHYT_RS28540
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 1,957,266 - +1.6 1,957,837 - BPHYT_RS28530 0.74 -0.3 1,958,100 + -1.2 1,958,466 - BPHYT_RS28535 0.57 -1.1 1,958,749 - +0.3 1,958,800 + -0.2 1,958,800 + +0.7 1,958,803 - -0.0 1,959,017 - BPHYT_RS28540 0.24 +0.8 1,959,017 - +1.7 1,959,250 - BPHYT_RS28540 0.44 +0.3 1,959,264 + BPHYT_RS28540 0.45 -0.7 1,959,373 - BPHYT_RS28540 0.54 +0.8 1,959,453 + BPHYT_RS28540 0.61 +0.8 1,959,472 - BPHYT_RS28540 0.63 +3.2 1,959,546 + BPHYT_RS28540 0.69 -0.2 1,959,652 + BPHYT_RS28540 0.79 -0.8
Or see this region's nucleotide sequence