Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28095 and BPHYT_RS28100 are separated by 25 nucleotides BPHYT_RS28100 and BPHYT_RS28105 are separated by 55 nucleotides BPHYT_RS28105 and BPHYT_RS28110 overlap by 28 nucleotides BPHYT_RS28110 and BPHYT_RS28115 are separated by 19 nucleotides BPHYT_RS28115 and BPHYT_RS28120 are separated by 578 nucleotides
BPHYT_RS28095: BPHYT_RS28095 - membrane protein, at 1,866,268 to 1,866,462
_RS28095
BPHYT_RS28100: BPHYT_RS28100 - hypothetical protein, at 1,866,488 to 1,866,682
_RS28100
BPHYT_RS28105: BPHYT_RS28105 - hypothetical protein, at 1,866,738 to 1,867,382
_RS28105
BPHYT_RS28110: BPHYT_RS28110 - hypothetical protein, at 1,867,355 to 1,867,555
_RS28110
BPHYT_RS28115: BPHYT_RS28115 - hypothetical protein, at 1,867,575 to 1,867,793
_RS28115
BPHYT_RS28120: BPHYT_RS28120 - hypothetical protein, at 1,868,372 to 1,868,653
_RS28120
Position (kb)
1867
1868 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1866.575 kb on - strand, within BPHYT_RS28100 at 1866.591 kb on + strand, within BPHYT_RS28100 at 1866.648 kb on + strand, within BPHYT_RS28100 at 1866.649 kb on - strand, within BPHYT_RS28100 at 1866.773 kb on - strand at 1866.811 kb on + strand, within BPHYT_RS28105 at 1866.935 kb on - strand, within BPHYT_RS28105 at 1867.425 kb on + strand, within BPHYT_RS28110 at 1867.558 kb on - strand at 1867.558 kb on - strand at 1867.662 kb on + strand, within BPHYT_RS28115 at 1867.965 kb on + strand at 1867.975 kb on + strand at 1868.352 kb on + strand at 1868.355 kb on + strand at 1868.393 kb on + strand at 1868.393 kb on + strand at 1868.394 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 1,866,575 - BPHYT_RS28100 0.45 -1.3 1,866,591 + BPHYT_RS28100 0.53 +0.6 1,866,648 + BPHYT_RS28100 0.82 +0.1 1,866,649 - BPHYT_RS28100 0.83 +0.3 1,866,773 - +0.8 1,866,811 + BPHYT_RS28105 0.11 +2.1 1,866,935 - BPHYT_RS28105 0.31 +0.4 1,867,425 + BPHYT_RS28110 0.35 -1.3 1,867,558 - -2.7 1,867,558 - -0.4 1,867,662 + BPHYT_RS28115 0.40 +0.3 1,867,965 + -2.8 1,867,975 + -0.7 1,868,352 + +0.5 1,868,355 + +0.2 1,868,393 + -0.6 1,868,393 + +0.6 1,868,394 - -0.1
Or see this region's nucleotide sequence