Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS27890

Experiment: 36hr PHYTO4 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS27880 and BPHYT_RS27890 are separated by 820 nucleotidesBPHYT_RS27890 and BPHYT_RS27895 are separated by 42 nucleotidesBPHYT_RS27895 and BPHYT_RS27900 are separated by 172 nucleotides BPHYT_RS27880: BPHYT_RS27880 - diguanylate phosphodiesterase, at 1,818,252 to 1,821,431 _RS27880 BPHYT_RS27890: BPHYT_RS27890 - hypothetical protein, at 1,822,252 to 1,822,512 _RS27890 BPHYT_RS27895: BPHYT_RS27895 - enoyl-CoA hydratase, at 1,822,555 to 1,822,998 _RS27895 BPHYT_RS27900: BPHYT_RS27900 - 2-hydroxyacid dehydrogenase, at 1,823,171 to 1,824,160 _RS27900 Position (kb) 1822 1823Strain fitness (log2 ratio) -1 0 1 2at 1821.283 kb on + strandat 1821.570 kb on - strandat 1822.417 kb on + strand, within BPHYT_RS27890at 1822.533 kb on + strandat 1822.589 kb on - strandat 1822.643 kb on + strand, within BPHYT_RS27895at 1822.853 kb on + strand, within BPHYT_RS27895at 1822.854 kb on - strand, within BPHYT_RS27895at 1823.004 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1
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1,821,283 + -1.3
1,821,570 - -0.8
1,822,417 + BPHYT_RS27890 0.63 +1.8
1,822,533 + +0.4
1,822,589 - +0.3
1,822,643 + BPHYT_RS27895 0.20 -0.1
1,822,853 + BPHYT_RS27895 0.67 +0.1
1,822,854 - BPHYT_RS27895 0.67 +0.0
1,823,004 + -0.7

Or see this region's nucleotide sequence