Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS27445

Experiment: 36hr PHYTO4 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS27440 and BPHYT_RS27445 are separated by 410 nucleotidesBPHYT_RS27445 and BPHYT_RS27450 are separated by 120 nucleotides BPHYT_RS27440: BPHYT_RS27440 - type II citrate synthase, at 1,732,162 to 1,733,460 _RS27440 BPHYT_RS27445: BPHYT_RS27445 - hypothetical protein, at 1,733,871 to 1,734,272 _RS27445 BPHYT_RS27450: BPHYT_RS27450 - alpha/beta hydrolase, at 1,734,393 to 1,735,196 _RS27450 Position (kb) 1733 1734 1735Strain fitness (log2 ratio) -1 0 1 2at 1732.916 kb on + strand, within BPHYT_RS27440at 1732.922 kb on + strand, within BPHYT_RS27440at 1732.923 kb on - strand, within BPHYT_RS27440at 1733.189 kb on + strand, within BPHYT_RS27440at 1733.275 kb on - strand, within BPHYT_RS27440at 1733.276 kb on + strand, within BPHYT_RS27440at 1733.462 kb on + strandat 1734.006 kb on - strand, within BPHYT_RS27445at 1734.121 kb on - strand, within BPHYT_RS27445at 1734.271 kb on - strandat 1734.271 kb on - strandat 1734.543 kb on - strand, within BPHYT_RS27450at 1734.622 kb on + strand, within BPHYT_RS27450at 1734.623 kb on - strand, within BPHYT_RS27450at 1735.189 kb on + strandat 1735.267 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1
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1,732,916 + BPHYT_RS27440 0.58 +0.3
1,732,922 + BPHYT_RS27440 0.59 +2.6
1,732,923 - BPHYT_RS27440 0.59 +0.6
1,733,189 + BPHYT_RS27440 0.79 +0.4
1,733,275 - BPHYT_RS27440 0.86 -0.8
1,733,276 + BPHYT_RS27440 0.86 -1.1
1,733,462 + +0.5
1,734,006 - BPHYT_RS27445 0.34 -1.2
1,734,121 - BPHYT_RS27445 0.62 -1.3
1,734,271 - -0.8
1,734,271 - +2.7
1,734,543 - BPHYT_RS27450 0.19 +0.8
1,734,622 + BPHYT_RS27450 0.28 -1.2
1,734,623 - BPHYT_RS27450 0.29 +0.6
1,735,189 + +0.6
1,735,267 - -1.0

Or see this region's nucleotide sequence