Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS27395

Experiment: 36hr PHYTO4 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS27390 and BPHYT_RS27395 are separated by 25 nucleotidesBPHYT_RS27395 and BPHYT_RS27400 are separated by 68 nucleotides BPHYT_RS27390: BPHYT_RS27390 - hydroxyacid dehydrogenase, at 1,718,647 to 1,719,708 _RS27390 BPHYT_RS27395: BPHYT_RS27395 - N-acetyltransferase GCN5, at 1,719,734 to 1,720,636 _RS27395 BPHYT_RS27400: BPHYT_RS27400 - D-amino acid oxidase, at 1,720,705 to 1,722,030 _RS27400 Position (kb) 1719 1720 1721Strain fitness (log2 ratio) -2 -1 0 1at 1718.758 kb on + strand, within BPHYT_RS27390at 1719.142 kb on + strand, within BPHYT_RS27390at 1719.143 kb on - strand, within BPHYT_RS27390at 1719.661 kb on - strandat 1719.837 kb on + strand, within BPHYT_RS27395at 1721.504 kb on - strand, within BPHYT_RS27400

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1
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1,718,758 + BPHYT_RS27390 0.10 +0.3
1,719,142 + BPHYT_RS27390 0.47 +0.6
1,719,143 - BPHYT_RS27390 0.47 +0.1
1,719,661 - -0.3
1,719,837 + BPHYT_RS27395 0.11 +0.8
1,721,504 - BPHYT_RS27400 0.60 -2.1

Or see this region's nucleotide sequence