Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26820 and BPHYT_RS26825 are separated by 131 nucleotides BPHYT_RS26825 and BPHYT_RS26830 are separated by 77 nucleotides BPHYT_RS26830 and BPHYT_RS26835 are separated by 83 nucleotides
BPHYT_RS26820: BPHYT_RS26820 - LysR family transcriptional regulator, at 1,589,300 to 1,590,187
_RS26820
BPHYT_RS26825: BPHYT_RS26825 - quercetin 2,3-dioxygenase, at 1,590,319 to 1,591,044
_RS26825
BPHYT_RS26830: BPHYT_RS26830 - membrane protein, at 1,591,122 to 1,591,535
_RS26830
BPHYT_RS26835: BPHYT_RS26835 - alkane 1-monooxygenase, at 1,591,619 to 1,592,773
_RS26835
Position (kb) 1590
1591
1592 Strain fitness (log2 ratio) -2
-1
0
1 at 1589.383 kb on - strand at 1589.616 kb on + strand, within BPHYT_RS26820 at 1589.679 kb on + strand, within BPHYT_RS26820 at 1590.139 kb on + strand at 1590.254 kb on + strand at 1590.255 kb on - strand at 1590.628 kb on + strand, within BPHYT_RS26825 at 1590.629 kb on - strand, within BPHYT_RS26825 at 1590.815 kb on - strand, within BPHYT_RS26825 at 1591.063 kb on + strand at 1591.063 kb on + strand at 1591.064 kb on - strand at 1591.130 kb on + strand at 1591.443 kb on - strand, within BPHYT_RS26830 at 1591.443 kb on - strand, within BPHYT_RS26830 at 1591.443 kb on - strand, within BPHYT_RS26830 at 1591.443 kb on - strand, within BPHYT_RS26830 at 1591.455 kb on + strand, within BPHYT_RS26830 at 1591.515 kb on + strand at 1591.744 kb on - strand, within BPHYT_RS26835 at 1592.004 kb on + strand, within BPHYT_RS26835
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 1,589,383 - -2.3 1,589,616 + BPHYT_RS26820 0.36 -2.7 1,589,679 + BPHYT_RS26820 0.43 -0.5 1,590,139 + -0.8 1,590,254 + -2.1 1,590,255 - +0.3 1,590,628 + BPHYT_RS26825 0.43 -0.5 1,590,629 - BPHYT_RS26825 0.43 -0.1 1,590,815 - BPHYT_RS26825 0.68 -1.2 1,591,063 + +1.2 1,591,063 + -1.0 1,591,064 - +1.2 1,591,130 + -1.5 1,591,443 - BPHYT_RS26830 0.78 +0.7 1,591,443 - BPHYT_RS26830 0.78 +1.0 1,591,443 - BPHYT_RS26830 0.78 -1.7 1,591,443 - BPHYT_RS26830 0.78 -2.6 1,591,455 + BPHYT_RS26830 0.80 -0.6 1,591,515 + -0.3 1,591,744 - BPHYT_RS26835 0.11 -0.6 1,592,004 + BPHYT_RS26835 0.33 -1.4
Or see this region's nucleotide sequence