Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25815

Experiment: 36hr PHYTO4 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25810 and BPHYT_RS25815 are separated by 16 nucleotidesBPHYT_RS25815 and BPHYT_RS25820 are separated by 252 nucleotides BPHYT_RS25810: BPHYT_RS25810 - acetaldehyde dehydrogenase (EC 1.2.1.3) (from data), at 1,363,169 to 1,365,559 _RS25810 BPHYT_RS25815: BPHYT_RS25815 - deoxyribose-phosphate aldolase, at 1,365,576 to 1,366,586 _RS25815 BPHYT_RS25820: BPHYT_RS25820 - sugar ABC transporter, at 1,366,839 to 1,368,461 _RS25820 Position (kb) 1365 1366 1367Strain fitness (log2 ratio) -1 0 1at 1365.236 kb on - strand, within BPHYT_RS25810at 1366.048 kb on + strand, within BPHYT_RS25815at 1366.223 kb on - strand, within BPHYT_RS25815at 1366.644 kb on - strandat 1367.044 kb on - strand, within BPHYT_RS25820at 1367.463 kb on + strand, within BPHYT_RS25820at 1367.464 kb on - strand, within BPHYT_RS25820

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1
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1,365,236 - BPHYT_RS25810 0.86 +0.4
1,366,048 + BPHYT_RS25815 0.47 -1.1
1,366,223 - BPHYT_RS25815 0.64 -0.1
1,366,644 - +0.2
1,367,044 - BPHYT_RS25820 0.13 +0.9
1,367,463 + BPHYT_RS25820 0.38 -0.2
1,367,464 - BPHYT_RS25820 0.39 -0.6

Or see this region's nucleotide sequence