Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25400

Experiment: 36hr PHYTO4 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25395 and BPHYT_RS25400 are separated by 177 nucleotidesBPHYT_RS25400 and BPHYT_RS25405 are separated by 77 nucleotides BPHYT_RS25395: BPHYT_RS25395 - 3-oxoacyl-ACP reductase, at 1,274,823 to 1,275,725 _RS25395 BPHYT_RS25400: BPHYT_RS25400 - butyryl-CoA dehydrogenase, at 1,275,903 to 1,277,048 _RS25400 BPHYT_RS25405: BPHYT_RS25405 - CoA-transferase, at 1,277,126 to 1,278,196 _RS25405 Position (kb) 1275 1276 1277 1278Strain fitness (log2 ratio) -2 -1 0 1at 1275.292 kb on - strand, within BPHYT_RS25395at 1275.315 kb on + strand, within BPHYT_RS25395at 1275.723 kb on + strandat 1275.724 kb on - strandat 1275.724 kb on - strandat 1275.956 kb on - strandat 1276.012 kb on + strandat 1276.104 kb on - strand, within BPHYT_RS25400at 1276.597 kb on + strand, within BPHYT_RS25400at 1277.218 kb on - strandat 1277.686 kb on - strand, within BPHYT_RS25405at 1277.785 kb on - strand, within BPHYT_RS25405at 1277.850 kb on + strand, within BPHYT_RS25405

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1
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1,275,292 - BPHYT_RS25395 0.52 +0.0
1,275,315 + BPHYT_RS25395 0.54 +0.7
1,275,723 + -1.2
1,275,724 - -0.0
1,275,724 - +0.0
1,275,956 - +0.0
1,276,012 + -0.8
1,276,104 - BPHYT_RS25400 0.18 -1.2
1,276,597 + BPHYT_RS25400 0.61 -2.1
1,277,218 - -2.0
1,277,686 - BPHYT_RS25405 0.52 -0.0
1,277,785 - BPHYT_RS25405 0.62 -1.2
1,277,850 + BPHYT_RS25405 0.68 +0.7

Or see this region's nucleotide sequence