Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25215

Experiment: 36hr PHYTO4 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25205 and BPHYT_RS25210 overlap by 4 nucleotidesBPHYT_RS25210 and BPHYT_RS25215 are separated by 73 nucleotidesBPHYT_RS25215 and BPHYT_RS25220 are separated by 33 nucleotides BPHYT_RS25205: BPHYT_RS25205 - electron transfer flavoprotein subunit alpha, at 1,228,764 to 1,229,945 _RS25205 BPHYT_RS25210: BPHYT_RS25210 - drug:proton antiporter, at 1,229,942 to 1,230,718 _RS25210 BPHYT_RS25215: BPHYT_RS25215 - (Fe-S)-binding protein, at 1,230,792 to 1,232,105 _RS25215 BPHYT_RS25220: BPHYT_RS25220 - ferredoxin oxidoreductase, at 1,232,139 to 1,233,386 _RS25220 Position (kb) 1230 1231 1232 1233Strain fitness (log2 ratio) -1 0 1at 1230.455 kb on + strand, within BPHYT_RS25210at 1232.412 kb on + strand, within BPHYT_RS25220at 1232.666 kb on + strand, within BPHYT_RS25220at 1232.895 kb on - strand, within BPHYT_RS25220

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1
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1,230,455 + BPHYT_RS25210 0.66 +0.4
1,232,412 + BPHYT_RS25220 0.22 -1.7
1,232,666 + BPHYT_RS25220 0.42 -1.7
1,232,895 - BPHYT_RS25220 0.61 +0.0

Or see this region's nucleotide sequence