Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24745 and BPHYT_RS24750 are separated by 280 nucleotides BPHYT_RS24750 and BPHYT_RS24755 are separated by 111 nucleotides BPHYT_RS24755 and BPHYT_RS24760 overlap by 4 nucleotides BPHYT_RS24760 and BPHYT_RS24765 are separated by 7 nucleotides
BPHYT_RS24745: BPHYT_RS24745 - transcriptional regulator, at 1,117,270 to 1,117,992
_RS24745
BPHYT_RS24750: BPHYT_RS24750 - hypothetical protein, at 1,118,273 to 1,118,542
_RS24750
BPHYT_RS24755: BPHYT_RS24755 - catalase, at 1,118,654 to 1,119,742
_RS24755
BPHYT_RS24760: BPHYT_RS24760 - cytochrome B561, at 1,119,739 to 1,120,278
_RS24760
BPHYT_RS24765: BPHYT_RS24765 - FAD-dependent oxidoreductase, at 1,120,286 to 1,122,781
_RS24765
Position (kb) 1118
1119
1120 Strain fitness (log2 ratio) -2
-1
0
1 at 1117.848 kb on - strand, within BPHYT_RS24745 at 1118.051 kb on + strand at 1118.402 kb on + strand, within BPHYT_RS24750 at 1118.440 kb on - strand, within BPHYT_RS24750 at 1118.596 kb on - strand at 1119.264 kb on - strand, within BPHYT_RS24755 at 1119.264 kb on - strand, within BPHYT_RS24755 at 1119.267 kb on - strand, within BPHYT_RS24755 at 1120.018 kb on + strand, within BPHYT_RS24760 at 1120.091 kb on - strand, within BPHYT_RS24760 at 1120.276 kb on + strand at 1120.277 kb on - strand at 1120.318 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 1,117,848 - BPHYT_RS24745 0.80 -1.9 1,118,051 + -1.7 1,118,402 + BPHYT_RS24750 0.48 -2.0 1,118,440 - BPHYT_RS24750 0.62 -0.3 1,118,596 - -2.1 1,119,264 - BPHYT_RS24755 0.56 -1.7 1,119,264 - BPHYT_RS24755 0.56 -1.6 1,119,267 - BPHYT_RS24755 0.56 -0.9 1,120,018 + BPHYT_RS24760 0.52 +0.0 1,120,091 - BPHYT_RS24760 0.65 +1.0 1,120,276 + +1.0 1,120,277 - +0.6 1,120,318 - -0.0
Or see this region's nucleotide sequence