Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS23460

Experiment: 36hr PHYTO4 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS23455 and BPHYT_RS23460 are separated by 46 nucleotidesBPHYT_RS23460 and BPHYT_RS23465 are separated by 213 nucleotides BPHYT_RS23455: BPHYT_RS23455 - phosphorylase, at 830,198 to 831,106 _RS23455 BPHYT_RS23460: BPHYT_RS23460 - AraC family transcriptional regulator, at 831,153 to 832,109 _RS23460 BPHYT_RS23465: BPHYT_RS23465 - aliphatic nitrilase, at 832,323 to 833,309 _RS23465 Position (kb) 831 832 833Strain fitness (log2 ratio) -1 0 1at 830.195 kb on + strandat 830.785 kb on + strand, within BPHYT_RS23455at 831.282 kb on - strand, within BPHYT_RS23460at 832.110 kb on - strandat 832.110 kb on - strandat 832.253 kb on + strandat 832.371 kb on + strandat 832.372 kb on - strandat 832.470 kb on + strand, within BPHYT_RS23465at 832.471 kb on - strand, within BPHYT_RS23465at 832.471 kb on - strand, within BPHYT_RS23465at 832.648 kb on - strand, within BPHYT_RS23465at 833.015 kb on + strand, within BPHYT_RS23465at 833.085 kb on + strand, within BPHYT_RS23465at 833.085 kb on + strand, within BPHYT_RS23465

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1
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830,195 + -0.5
830,785 + BPHYT_RS23455 0.65 -0.4
831,282 - BPHYT_RS23460 0.13 -0.5
832,110 - +0.6
832,110 - +1.5
832,253 + +0.4
832,371 + -0.6
832,372 - -0.2
832,470 + BPHYT_RS23465 0.15 -0.2
832,471 - BPHYT_RS23465 0.15 +1.3
832,471 - BPHYT_RS23465 0.15 -1.4
832,648 - BPHYT_RS23465 0.33 +1.1
833,015 + BPHYT_RS23465 0.70 -0.5
833,085 + BPHYT_RS23465 0.77 +1.1
833,085 + BPHYT_RS23465 0.77 -0.2

Or see this region's nucleotide sequence