Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23310 and BPHYT_RS23315 are separated by 82 nucleotides BPHYT_RS23315 and BPHYT_RS23320 are separated by 123 nucleotides BPHYT_RS23320 and BPHYT_RS23325 are separated by 8 nucleotides BPHYT_RS23325 and BPHYT_RS23330 are separated by 400 nucleotides
BPHYT_RS23310: BPHYT_RS23310 - hypothetical protein, at 791,746 to 792,558
_RS23310
BPHYT_RS23315: BPHYT_RS23315 - hypothetical protein, at 792,641 to 793,042
_RS23315
BPHYT_RS23320: BPHYT_RS23320 - hypothetical protein, at 793,166 to 793,630
_RS23320
BPHYT_RS23325: BPHYT_RS23325 - ribonuclease, at 793,639 to 794,094
_RS23325
BPHYT_RS23330: BPHYT_RS23330 - hypothetical protein, at 794,495 to 794,680
_RS23330
Position (kb) 793
794 Strain fitness (log2 ratio) -1
0
1
2 at 792.181 kb on + strand, within BPHYT_RS23310 at 792.218 kb on - strand, within BPHYT_RS23310 at 792.590 kb on + strand at 792.613 kb on + strand at 792.897 kb on - strand, within BPHYT_RS23315 at 793.137 kb on + strand at 793.335 kb on + strand, within BPHYT_RS23320 at 793.435 kb on + strand, within BPHYT_RS23320 at 793.470 kb on - strand, within BPHYT_RS23320 at 793.477 kb on - strand, within BPHYT_RS23320 at 793.481 kb on + strand, within BPHYT_RS23320 at 793.617 kb on + strand at 793.641 kb on - strand at 793.683 kb on - strand at 793.721 kb on - strand, within BPHYT_RS23325 at 793.721 kb on - strand, within BPHYT_RS23325 at 793.769 kb on + strand, within BPHYT_RS23325 at 793.770 kb on - strand, within BPHYT_RS23325 at 794.138 kb on - strand at 794.138 kb on - strand at 794.144 kb on + strand at 794.387 kb on + strand at 794.405 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 792,181 + BPHYT_RS23310 0.54 -0.3 792,218 - BPHYT_RS23310 0.58 -1.2 792,590 + +0.2 792,613 + +0.3 792,897 - BPHYT_RS23315 0.64 -1.0 793,137 + -0.1 793,335 + BPHYT_RS23320 0.36 -0.2 793,435 + BPHYT_RS23320 0.58 +1.1 793,470 - BPHYT_RS23320 0.65 -0.9 793,477 - BPHYT_RS23320 0.67 +1.3 793,481 + BPHYT_RS23320 0.68 +0.7 793,617 + -1.0 793,641 - -0.5 793,683 - -1.0 793,721 - BPHYT_RS23325 0.18 -0.5 793,721 - BPHYT_RS23325 0.18 +2.3 793,769 + BPHYT_RS23325 0.29 +0.1 793,770 - BPHYT_RS23325 0.29 -0.3 794,138 - -0.5 794,138 - +0.2 794,144 + -0.2 794,387 + +0.3 794,405 + -0.7
Or see this region's nucleotide sequence