Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS23145

Experiment: 36hr PHYTO4 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS23135 and BPHYT_RS23140 overlap by 4 nucleotidesBPHYT_RS23140 and BPHYT_RS23145 overlap by 4 nucleotidesBPHYT_RS23145 and BPHYT_RS23150 are separated by 212 nucleotides BPHYT_RS23135: BPHYT_RS23135 - putrescine/spermidine ABC transporter permease, at 756,182 to 757,093 _RS23135 BPHYT_RS23140: BPHYT_RS23140 - putrescine/spermidine ABC transporter permease, at 757,090 to 758,064 _RS23140 BPHYT_RS23145: BPHYT_RS23145 - putrescine/spermidine ABC transporter ATP-binding protein, at 758,061 to 759,224 _RS23145 BPHYT_RS23150: BPHYT_RS23150 - ABC transporter substrate-binding protein, at 759,437 to 760,558 _RS23150 Position (kb) 758 759 760Strain fitness (log2 ratio) -1 0 1at 757.121 kb on + strandat 757.389 kb on - strand, within BPHYT_RS23140at 757.848 kb on - strand, within BPHYT_RS23140at 759.420 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1
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757,121 + +1.5
757,389 - BPHYT_RS23140 0.31 -0.6
757,848 - BPHYT_RS23140 0.78 +0.4
759,420 + -0.7

Or see this region's nucleotide sequence