Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22080 and BPHYT_RS22085 are separated by 12 nucleotides BPHYT_RS22085 and BPHYT_RS22090 are separated by 173 nucleotides BPHYT_RS22090 and BPHYT_RS22095 are separated by 224 nucleotides
BPHYT_RS22080: BPHYT_RS22080 - hypothetical protein, at 511,329 to 512,279
_RS22080
BPHYT_RS22085: BPHYT_RS22085 - transglutaminase, at 512,292 to 513,092
_RS22085
BPHYT_RS22090: BPHYT_RS22090 - hypothetical protein, at 513,266 to 514,138
_RS22090
BPHYT_RS22095: BPHYT_RS22095 - porin, at 514,363 to 515,544
_RS22095
Position (kb) 513
514
515 Strain fitness (log2 ratio) -2
-1
0
1
2 at 512.292 kb on - strand at 512.292 kb on - strand at 512.295 kb on + strand at 512.296 kb on - strand at 512.296 kb on - strand at 512.323 kb on - strand at 512.715 kb on + strand, within BPHYT_RS22085 at 512.716 kb on - strand, within BPHYT_RS22085 at 512.741 kb on + strand, within BPHYT_RS22085 at 512.742 kb on - strand, within BPHYT_RS22085 at 512.742 kb on - strand, within BPHYT_RS22085 at 512.780 kb on - strand, within BPHYT_RS22085 at 512.842 kb on - strand, within BPHYT_RS22085 at 512.853 kb on + strand, within BPHYT_RS22085 at 512.854 kb on - strand, within BPHYT_RS22085 at 512.997 kb on + strand, within BPHYT_RS22085 at 513.121 kb on + strand at 513.121 kb on + strand at 513.122 kb on - strand at 513.122 kb on - strand at 513.122 kb on - strand at 513.149 kb on + strand at 513.191 kb on + strand at 513.191 kb on + strand at 513.191 kb on + strand at 513.194 kb on - strand at 513.194 kb on - strand at 513.194 kb on - strand at 513.265 kb on + strand at 513.272 kb on + strand at 513.272 kb on + strand at 513.272 kb on + strand at 513.272 kb on + strand at 513.273 kb on - strand at 513.273 kb on - strand at 513.394 kb on + strand, within BPHYT_RS22090 at 513.395 kb on - strand, within BPHYT_RS22090 at 513.609 kb on + strand, within BPHYT_RS22090 at 513.654 kb on - strand, within BPHYT_RS22090 at 513.696 kb on - strand, within BPHYT_RS22090 at 513.855 kb on - strand, within BPHYT_RS22090 at 513.855 kb on - strand, within BPHYT_RS22090 at 513.878 kb on - strand, within BPHYT_RS22090 at 513.911 kb on + strand, within BPHYT_RS22090 at 513.911 kb on + strand, within BPHYT_RS22090 at 514.136 kb on + strand at 514.422 kb on - strand at 514.428 kb on - strand at 514.474 kb on - strand at 514.474 kb on - strand at 514.515 kb on - strand, within BPHYT_RS22095 at 514.541 kb on + strand, within BPHYT_RS22095 at 514.568 kb on + strand, within BPHYT_RS22095 at 514.602 kb on - strand, within BPHYT_RS22095 at 514.602 kb on - strand, within BPHYT_RS22095 at 514.607 kb on + strand, within BPHYT_RS22095 at 514.607 kb on + strand, within BPHYT_RS22095 at 514.607 kb on + strand, within BPHYT_RS22095 at 514.608 kb on - strand, within BPHYT_RS22095 at 514.608 kb on - strand, within BPHYT_RS22095 at 514.652 kb on + strand, within BPHYT_RS22095 at 514.670 kb on + strand, within BPHYT_RS22095 at 514.671 kb on - strand, within BPHYT_RS22095 at 514.713 kb on - strand, within BPHYT_RS22095 at 514.760 kb on + strand, within BPHYT_RS22095 at 514.806 kb on - strand, within BPHYT_RS22095 at 514.917 kb on - strand, within BPHYT_RS22095 at 514.949 kb on + strand, within BPHYT_RS22095 at 514.950 kb on - strand, within BPHYT_RS22095 at 515.012 kb on + strand, within BPHYT_RS22095 at 515.012 kb on + strand at 515.013 kb on - strand, within BPHYT_RS22095 at 515.013 kb on - strand, within BPHYT_RS22095 at 515.013 kb on - strand, within BPHYT_RS22095 at 515.054 kb on + strand, within BPHYT_RS22095 at 515.055 kb on - strand, within BPHYT_RS22095
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 512,292 - -0.4 512,292 - -2.5 512,295 + -0.6 512,296 - -0.1 512,296 - +0.3 512,323 - +2.2 512,715 + BPHYT_RS22085 0.53 +0.7 512,716 - BPHYT_RS22085 0.53 -1.0 512,741 + BPHYT_RS22085 0.56 -0.6 512,742 - BPHYT_RS22085 0.56 -0.4 512,742 - BPHYT_RS22085 0.56 +0.2 512,780 - BPHYT_RS22085 0.61 +2.1 512,842 - BPHYT_RS22085 0.69 -1.0 512,853 + BPHYT_RS22085 0.70 -0.7 512,854 - BPHYT_RS22085 0.70 +0.9 512,997 + BPHYT_RS22085 0.88 +0.1 513,121 + +0.2 513,121 + -2.0 513,122 - -0.6 513,122 - +0.1 513,122 - +0.1 513,149 + +0.5 513,191 + +1.2 513,191 + -1.1 513,191 + +0.4 513,194 - +1.4 513,194 - -1.2 513,194 - +0.8 513,265 + -1.5 513,272 + +0.0 513,272 + -0.2 513,272 + -1.8 513,272 + +0.1 513,273 - +0.1 513,273 - +0.7 513,394 + BPHYT_RS22090 0.15 +0.8 513,395 - BPHYT_RS22090 0.15 +0.2 513,609 + BPHYT_RS22090 0.39 -0.1 513,654 - BPHYT_RS22090 0.44 +0.5 513,696 - BPHYT_RS22090 0.49 -0.9 513,855 - BPHYT_RS22090 0.67 +0.1 513,855 - BPHYT_RS22090 0.67 +0.3 513,878 - BPHYT_RS22090 0.70 -1.1 513,911 + BPHYT_RS22090 0.74 +0.3 513,911 + BPHYT_RS22090 0.74 +1.0 514,136 + -0.1 514,422 - -0.5 514,428 - -0.7 514,474 - +0.4 514,474 - +0.4 514,515 - BPHYT_RS22095 0.13 -1.3 514,541 + BPHYT_RS22095 0.15 +1.0 514,568 + BPHYT_RS22095 0.17 -0.7 514,602 - BPHYT_RS22095 0.20 -0.5 514,602 - BPHYT_RS22095 0.20 -0.2 514,607 + BPHYT_RS22095 0.21 +0.6 514,607 + BPHYT_RS22095 0.21 -0.0 514,607 + BPHYT_RS22095 0.21 -0.3 514,608 - BPHYT_RS22095 0.21 -0.4 514,608 - BPHYT_RS22095 0.21 -2.6 514,652 + BPHYT_RS22095 0.24 +1.0 514,670 + BPHYT_RS22095 0.26 -0.2 514,671 - BPHYT_RS22095 0.26 -2.6 514,713 - BPHYT_RS22095 0.30 +0.2 514,760 + BPHYT_RS22095 0.34 +0.4 514,806 - BPHYT_RS22095 0.37 -1.5 514,917 - BPHYT_RS22095 0.47 -0.5 514,949 + BPHYT_RS22095 0.50 +2.3 514,950 - BPHYT_RS22095 0.50 -0.9 515,012 + BPHYT_RS22095 0.55 +0.1 515,012 + +1.0 515,013 - BPHYT_RS22095 0.55 +0.3 515,013 - BPHYT_RS22095 0.55 +1.6 515,013 - BPHYT_RS22095 0.55 +0.9 515,054 + BPHYT_RS22095 0.58 -1.5 515,055 - BPHYT_RS22095 0.59 -0.6
Or see this region's nucleotide sequence