Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21615 and BPHYT_RS21620 overlap by 4 nucleotides BPHYT_RS21620 and BPHYT_RS21625 are separated by 117 nucleotides BPHYT_RS21625 and BPHYT_RS21630 are separated by 184 nucleotides
BPHYT_RS21615: BPHYT_RS21615 - FMN-dependent NADH-azoreductase, at 405,979 to 406,653
_RS21615
BPHYT_RS21620: BPHYT_RS21620 - GCN5 family acetyltransferase, at 406,650 to 407,117
_RS21620
BPHYT_RS21625: BPHYT_RS21625 - LysR family transcriptional regulator, at 407,235 to 408,149
_RS21625
BPHYT_RS21630: BPHYT_RS21630 - NmrA family transcriptional regulator, at 408,334 to 409,197
_RS21630
Position (kb) 407
408
409 Strain fitness (log2 ratio) -2
-1
0
1
2 at 406.735 kb on + strand, within BPHYT_RS21620 at 407.320 kb on + strand at 407.321 kb on - strand at 407.623 kb on + strand, within BPHYT_RS21625 at 408.109 kb on - strand at 408.215 kb on - strand at 408.308 kb on - strand at 408.308 kb on - strand at 408.311 kb on + strand at 408.312 kb on - strand at 408.315 kb on + strand at 408.315 kb on + strand at 409.053 kb on + strand, within BPHYT_RS21630
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 406,735 + BPHYT_RS21620 0.18 -0.3 407,320 + -1.4 407,321 - -1.6 407,623 + BPHYT_RS21625 0.42 -2.0 408,109 - -0.7 408,215 - -0.4 408,308 - -1.1 408,308 - +2.1 408,311 + -1.3 408,312 - +0.1 408,315 + -1.7 408,315 + +0.3 409,053 + BPHYT_RS21630 0.83 +0.3
Or see this region's nucleotide sequence